Pochechuev M S, Fedotov I V, Ivashkina O I, Roshchina M A, Anokhin K V, Zheltikov A M
Physics Department, International Laser Center, M.V. Lomonosov Moscow State University, Moscow, 119992, Russia.
Kurchatov Institute National Research Center, Moscow, Russia.
J Biophotonics. 2018 Jan;11(1). doi: 10.1002/jbio.201600203. Epub 2017 May 18.
Optical coupling between a single, individually addressable neuron and a properly designed optical fiber is demonstrated. Two-photon imaging is shown to enable a quantitative in situ analysis of such fiber-single-neuron coupling in the live brain of transgenic mice. Fiber-optic interrogation of single pyramidal neurons in mouse brain cortex is performed with the positioning of the fiber probe relative to the neuron accurately mapped by means of two-photon imaging. These results pave the way for fiber-optic interfaces to single neurons for a stimulation and interrogation of individually addressable brain cells in chronic in vivo studies on freely behaving transgenic animal models, as well as the integration of fiber-optic single-neuron stimulation into the optical imaging framework.
展示了单个可单独寻址的神经元与精心设计的光纤之间的光耦合。结果表明，双光子成像能够对转基因小鼠活体大脑中这种光纤-单神经元耦合进行定量原位分析。通过双光子成像精确绘制光纤探针相对于神经元的位置，对小鼠大脑皮层中的单个锥体神经元进行光纤检测。这些结果为在自由行为的转基因动物模型的慢性体内研究中，使用光纤接口连接单个神经元以刺激和检测可单独寻址的脑细胞，以及将光纤单神经元刺激集成到光学成像框架铺平了道路。
