Opt Lett. 2021 Mar 1;46(5):1093-1096. doi: 10.1364/OL.412760.
Visualizing activity patterns of distinct cell types during complex behaviors is essential to understand complex neural networks. It remains challenging to excite multiple fluorophores simultaneously so that different types of neurons can be imaged. In this Letter, we report a multicolor fiber-optic two-photon endomicroscopy platform in which two pulses from a Ti:sapphire laser and an optical parametric oscillator were synchronized and delivered through a single customized double-clad fiber to excite multiple chromophores. A third virtual wavelength could also be generated by spatial-temporal overlapping of the two pulses. The performance of the fiber-optic multicolor two-photon endomicroscope was demonstrated by in vivo imaging of a mouse cerebral cortex with "Brainbow" labeling.
在复杂行为中可视化不同细胞类型的活动模式对于理解复杂的神经网络至关重要。同时激发多个荧光团仍然具有挑战性，因此无法对不同类型的神经元进行成像。在本研究中，我们报告了一种多色光纤双光子内窥显微镜平台，该平台通过单根定制的双包层光纤同步传输来自钛宝石激光器和光参量振荡器的两个脉冲，从而激发多个发色团。两个脉冲的时空重叠也可以产生第三个虚拟波长。通过对“Brainbow”标记的小鼠大脑皮层进行体内成像，验证了光纤多色双光子内窥显微镜的性能。
