Ingersoll J D, Wylie D E
Norden Laboratories Inc., Lincoln, NE 68521.
Am J Vet Res. 1988 Sep;49(9):1472-9.
Serologic virus neutralization tests, indirect immunofluorescence tests, and ELISA, using tissue culture-adapted feline infectious peritonitis virus (FIPV) or feline enteric coronavirus (FECV) were compared for their ability to distinguish specific virus exposure in cats. Sera of specific-pathogen-free cats inoculated with virulent or modified FIPV or FECV were used to compare the sensitivity and specificity of the homologous assays to a heterologous assay that measures antibody reactivity with transmissible gastroenteritis virus of swine. The geometric means of the serologic titers in FIPV and FECV assays were higher for FIPV- or FECV-infected specific-pathogen-free cats than the geometric means of the transmissible gastroenteritis virus assays for most groups. None of the assays was specific enough to discern the virus to which a cat had been exposed. However, the FIPV virus neutralization test appeared to be more sensitive for detection of an early response to FIPV infection than did the FIPV immunofluorescence test or FIPV-ELISA.
比较了血清学病毒中和试验、间接免疫荧光试验以及使用适应组织培养的猫传染性腹膜炎病毒（FIPV）或猫肠道冠状病毒（FECV）的酶联免疫吸附测定（ELISA）区分猫特定病毒暴露的能力。使用接种了强毒或改良FIPV或FECV的无特定病原体猫的血清，比较同源试验与测量与猪传染性胃肠炎病毒抗体反应性的异源试验的敏感性和特异性。对于大多数组，FIPV或FECV感染的无特定病原体猫在FIPV和FECV试验中的血清学滴度几何平均值高于传染性胃肠炎病毒试验的几何平均值。没有一种试验特异性足够高，能够辨别猫所暴露的病毒。然而，FIPV病毒中和试验在检测对FIPV感染的早期反应方面似乎比FIPV免疫荧光试验或FIPV-ELISA更敏感。
