Hayashi J, Shimada Y, Tagami J, Sumi Y, Sadr A
1 Cariology and Operative Dentistry Department, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
2 Department of Operative Dentistry, Okayama University Graduate School of Medicine, Okayama, Japan.
J Dent Res. 2017 Aug;96(9):992-998. doi: 10.1177/0022034517709005. Epub 2017 May 18.
The aims of this study were to observe the behavior of composite and formation of gaps during and immediately after light polymerization using swept source optical coherence tomography (OCT) and to compare the interfacial integrity of adhesives in cavities through 3-dimensional (3D) image analysis. Forty tapered cylindrical cavities (4-mm diameter, 2-mm depth) were prepared in bovine incisors and restored using Bond Force (BF), Scotchbond Universal Adhesive (SBU), OptiBond XTR (XTR), or Clearfil SE Bond 2 (SE2), followed by Estelite Flow Quick flowable composite. Real-time imaging was performed at the center of restoration by the OCT system (laser center wavelength: 1,330 nm; frequency: 30 KHz) during and up to 10 min after light curing. The 3D scanning was performed 0, 1, 3, 5, and 10 min after light curing. The percentages of sealed enamel and dentin interface area (E%, D%) were calculated using Amira software. In real-time videos, the initial gaps appeared as a bright scattered area mainly on dentin floor and rapidly progressed along the cavity floor. The timing, rate, and extent of gap formation were different among the specimens. From 3D visualization, gap progress could be seen on both enamel and dentin even after irradiation; furthermore, typical toroidal gap patterns appeared at the dentin floor of BF and SBU. XTR and SE2 showed nearly perfect sealing performance on the dentin floor up to the 10 min that images were recorded. From quantitative analysis, SE2 and XTR showed significantly higher E% and D% than other groups. SBU showed the smallest E% and BF showed a significantly smaller D% than other groups ( P < 0.05). In conclusion, real-time observation of composite placement and 3D quantification of interfacial gaps were implemented within the experimental limitations. Interfacial gap formation during polymerization of the composite depended on the adhesive system used. The formed gaps continued to propagate after composite light curing finished.
本研究的目的是使用扫频光学相干断层扫描(OCT)观察光聚合过程中及光聚合后即刻复合树脂的行为和间隙形成情况,并通过三维(3D)图像分析比较窝洞内粘结剂的界面完整性。在牛切牙上制备40个锥形圆柱形窝洞(直径4mm,深度2mm),并用Bond Force(BF)、Scotchbond通用粘结剂(SBU)、OptiBond XTR(XTR)或Clearfil SE Bond 2(SE2)进行修复,随后使用Estelite Flow Quick流动复合树脂。在光固化期间及光固化后长达10分钟内,通过OCT系统(激光中心波长:1330nm;频率:30KHz)在修复体中心进行实时成像。在光固化后0、1、3、5和10分钟进行3D扫描。使用Amira软件计算密封釉质和牙本质界面面积的百分比(E%,D%)。在实时视频中,初始间隙主要表现为牙本质底部的明亮散射区域,并沿窝洞底部迅速扩展。不同标本间间隙形成的时间、速率和程度各不相同。从3D可视化观察,即使在照射后,釉质和牙本质上均可看到间隙扩展;此外,BF和SBU的牙本质底部出现典型的环形间隙模式。在记录图像的10分钟内,XTR和SE2在牙本质底部显示出近乎完美的密封性能。定量分析显示,SE2和XTR的E%和D%显著高于其他组。SBU的E%最小,BF的D%显著小于其他组(P<0.05)。总之,在实验限制范围内实现了复合树脂放置的实时观察和界面间隙的3D定量分析。复合树脂聚合过程中的界面间隙形成取决于所使用的粘结系统。复合树脂光固化完成后,形成的间隙仍继续扩展。