Ura Blendi, Scrimin Federica, Franchin Cinzia, Arrigoni Giorgio, Licastro Danilo, Monasta Lorenzo, Ricci Giuseppe
Institute for Maternal and Child Health, IRCCS 'Burlo Garofolo', I-34137 Trieste, Italy.
Department of Biomedical Sciences, University of Padova, I-35122 Padova, Italy.
Oncol Lett. 2017 May;13(5):3912-3920. doi: 10.3892/ol.2017.5943. Epub 2017 Mar 29.
Uterine leiomyoma is the most common female reproductive tract benign tumor. Little is known about protein composition and changes in the leiomyoma interstitial fluid (IF). The present study focused on changes in protein abundance in the IF of leiomyoma. Leiomyoma IFs and adjacent myometrial IFs were obtained and analyzed by two-dimensional electrophoresis (2-DE) coupled with mass spectrometry and western blotting for 2-DE data validation. A total of 25 unique proteins were observed to change significantly (P<0.05). Of these proteins with different abundance, 22 had not been previously identified in leiomyoma IF. analysis predicted that three of these proteins were secreted via classical mechanisms, while 22 were secreted via non-classical mechanisms. Ingenuity Pathway Analysis identified 17 proteins associated with cellular migration and proliferation. Among these, phosphoglycerate mutase 1 had not been previously associated with leiomyoma. The abundance of seven proteins was further validated by western blotting. A comparative proteomic approach identified a number of proteins associated with cellular migration and proliferation, with changes in abundance in IF likely to be involved in tumor development. Further studies will be required to investigate the role of these proteins in leiomyoma IF and their possible association with tumor development and growth.
子宫平滑肌瘤是女性生殖道最常见的良性肿瘤。关于平滑肌瘤间质液（IF）的蛋白质组成和变化知之甚少。本研究聚焦于平滑肌瘤IF中蛋白质丰度的变化。获取平滑肌瘤IF和相邻肌层IF，并通过二维电泳（2-DE）结合质谱分析以及蛋白质印迹法对2-DE数据进行验证。共观察到25种独特蛋白质有显著变化（P<0.05）。在这些丰度不同的蛋白质中，有22种此前未在平滑肌瘤IF中被鉴定出来。分析预测其中3种蛋白质通过经典机制分泌，而22种通过非经典机制分泌。 Ingenuity通路分析确定了17种与细胞迁移和增殖相关的蛋白质。其中，磷酸甘油酸变位酶1此前未与平滑肌瘤相关联。通过蛋白质印迹法进一步验证了7种蛋白质的丰度。一种比较蛋白质组学方法鉴定出许多与细胞迁移和增殖相关的蛋白质，IF中丰度的变化可能参与肿瘤发展。需要进一步研究来探究这些蛋白质在平滑肌瘤IF中的作用及其与肿瘤发展和生长的可能关联。
