苏丹传统医学中使用的阔叶非洲李树皮的抗氧化活性、乙酰胆碱酯酶抑制潜力及植物化学分析

Antioxidant activity, acetylcholinesterase inhibitory potential and phytochemical analysis of Sarcocephalus latifolius Sm. bark used in traditional medicine in Sudan.

作者信息

Osama Alsiddig, Awadelkarim Sufyan, Ali Amna

机构信息

Chemistry Department, Omdurman Islamic University, P.O. Box 382, Omdurman, Sudan.

Medical Biochemistry Research Unit, Medicinal and Aromatic Plants Research Institute, National Centre for Research, P.O. Box 2404, Khartoum, Sudan.

出版信息

BMC Complement Altern Med. 2017 May 18;17(1):270. doi: 10.1186/s12906-017-1772-6.

BACKGROUND

Sarcocephalus latifolius is used as a traditional medicine for curing many diseases in Sudan. The main objective of the current study was to determine the antioxidant activity and acetylcholinesterase inhibition (AChEI) of S. latifolius, and to estimate its total phenolic and flavonoid contents.

METHODS

Antioxidant activity of the tested plant extracts was carried out by determining their ability to scavenge the 2,2-diphenyl-1-picryl hydrazyl (DPPH) free radical. On the other hand, AChE inhibitory activity was determined spectrophotometrically using the Ellman's colorimetric method. The levels of total phenols and flavonoids were determined quantitatively using spectrophotometric methods. MTT assay was consumed to assess the cytotoxic effect of the most active fractions. These fractions were subjected to phytochemical analysis using GC-MS techniques to determine thier chemical composition.

RESULTS

Hexane and chloroform fractions exhibited the highest antioxidant activity with IC values of (0.098 ± 0.08 and 0.099 ± 0.029 mg/ml) respectively. Standard propyl gallate had the lowest IC value of 0.0414 ± 0.11 mg/ml. The ethanolic crude extract showed low AChEI activity with 40.2 ± 0.10%. High concentrations of phenolic and flavonoid contents were observed. GCMS revealed the presence of well-known antioxidants compounds e.g. Vitamin E and caffeic acid.

CONCLUSION

The ethanolic extract of bark of S. latifolius showed potent antioxidant effects and low AChEI activity, high phenolic and flavonoid contents and presence of pharmacologically active compounds. These findings explain its wide usages in traditional medicine.

背景

阔叶非洲李在苏丹被用作治疗多种疾病的传统药物。本研究的主要目的是测定阔叶非洲李的抗氧化活性和乙酰胆碱酯酶抑制作用（AChEI），并估算其总酚和黄酮含量。

方法

通过测定受试植物提取物清除2,2 - 二苯基 - 1 - 苦基肼（DPPH）自由基的能力来进行抗氧化活性检测。另一方面，采用Ellman比色法通过分光光度法测定乙酰胆碱酯酶抑制活性。使用分光光度法对总酚和黄酮水平进行定量测定。采用MTT法评估活性最强的组分的细胞毒性作用。使用气相色谱 - 质谱联用（GC - MS）技术对这些组分进行植物化学分析以确定其化学成分。

结果

己烷和氯仿组分表现出最高的抗氧化活性，IC值分别为（0.098±0.08和0.099±0.029毫克/毫升）。标准没食子酸丙酯的IC值最低，为0.0414±0.11毫克/毫升。乙醇粗提物显示出较低的乙酰胆碱酯酶抑制活性，为40.2±0.10%。观察到高浓度的酚类和黄酮类成分。气相色谱 - 质谱联用分析显示存在维生素E和咖啡酸等知名抗氧化化合物。