Prochowska S, Niżański W
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Pol J Vet Sci. 2017 Mar 28;20(1):19-24. doi: 10.1515/pjvs-2017-0003.
The aim of this study was to provide a comparative analysis of in vitro fertilizing potential of frozen-thawed urethral and epididymal feline spermatozoa. Both types of semen were collected from 7 cats and cryopreserved in liquid nitrogen. To perform in vitro fertilization, both urethral and epididymal samples from the same individual were thawed and spermatozoa were co-incubated with in vitro matured cat oocytes. Obtained embryos were cultured in vitro for 7 days in a commercial medium. Cleavage rate, morula rate and blastocyst rate were calculated. Experiment was run in 10 replicates. The examined parameters showed no significant differences between urethral and epididymal spermatozoa (p>0.05). Cleavage rate and embryo's development were highly variable between replicates, even for the different sperm samples collected from one individual. There was no significant correlation between fertilizing capacity of two types of spermatozoa collected from the same male. In this study we confirmed that cryopreserved urethral spermatozoa have equally good fertilizing potential as epididymal ones, and both can be successfully used for in vitro fertilization in cats with the use of commercial medium.
本研究的目的是对冷冻解冻后的猫尿道和附睾精子的体外受精潜力进行比较分析。两种类型的精液均取自7只猫，并在液氮中冷冻保存。为了进行体外受精，将来自同一个体的尿道和附睾样本解冻，并使精子与体外成熟的猫卵母细胞共同孵育。将获得的胚胎在商业培养基中体外培养7天。计算卵裂率、桑椹胚率和囊胚率。实验进行了10次重复。所检测的参数在尿道精子和附睾精子之间没有显著差异（p>0.05）。即使是从同一个体收集的不同精子样本，卵裂率和胚胎发育在重复实验之间也存在很大差异。从同一雄性收集的两种类型精子的受精能力之间没有显著相关性。在本研究中，我们证实冷冻保存的尿道精子与附睾精子具有同样良好的受精潜力，并且两者都可以成功地用于使用商业培养基的猫的体外受精。
