Castiblanco Valheria, Marulanda Jose J, Würschum Tobias, Miedaner Thomas
State Plant Breeding Institute, University of Hohenheim, 70593, Stuttgart, Germany.
Institute of Plant Breeding, Seed Science and Population Genetics, University of Hohenheim, 79593, Stuttgart, Germany.
BMC Genet. 2017 May 19;18(1):49. doi: 10.1186/s12863-017-0511-9.
Quantitative traits are common in nature, but quantitative pathogenicity has received only little attention in phytopathology. In this study, we used 100 Fusarium culmorum isolates collected from natural field environments to assess their variation for two quantitative traits, aggressiveness and deoxynivalenol (DON) production on wheat plants grown in four different field environments (location-year combinations). Seventeen Fusarium graminearum pathogenicity candidate genes were assessed for their effect on the aggressiveness and DON production of F. culmorum under field conditions.
For both traits, genotypic variance among isolates was high and significant while the isolate-by-environment interaction was also significant, amounting to approximately half of the genotypic variance. Among the studied candidate genes, the mitogen-activated protein kinase (MAPK) HOG1 was found to be significantly associated with aggressiveness and deoxynivalenol (DON) production, explaining 10.29 and 6.05% of the genotypic variance, respectively.
To the best of our knowledge, this is the first report of a protein kinase regulator explaining differences in field aggressiveness and mycotoxin production among individuals from natural populations of a plant pathogen.
数量性状在自然界中很常见，但在植物病理学中，数量致病性受到的关注较少。在本研究中，我们使用了从自然田间环境中收集的100株禾谷镰刀菌分离株，来评估它们在四种不同田间环境（地点-年份组合）下种植的小麦植株上的两个数量性状的变异情况，即致病性和脱氧雪腐镰刀菌烯醇（DON）产量。评估了17个禾谷镰刀菌致病性候选基因对田间条件下禾谷镰刀菌致病性和DON产量的影响。
对于这两个性状，分离株之间的基因型变异都很高且显著，而分离株与环境的相互作用也很显著，约占基因型变异的一半。在所研究的候选基因中，丝裂原活化蛋白激酶（MAPK）HOG1被发现与致病性和脱氧雪腐镰刀菌烯醇（DON）产量显著相关，分别解释了10.29%和6.05%的基因型变异。
据我们所知，这是首次报道一种蛋白激酶调节因子可解释植物病原菌自然群体中个体间田间致病性和霉菌毒素产生的差异。
