The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
The Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China.
Viruses. 2018 Aug 15;10(8):432. doi: 10.3390/v10080432.
Here, we characterized a negative single-stranded (-ss)RNA mycovirus, Botrytis cinerea mymonavirus 1 (BcMyV1), isolated from the phytopathogenic fungus . The genome of BcMyV1 is 7863 nt in length, possessing three open reading frames (ORF1⁻3). The ORF1 encodes a large polypeptide containing a conserved mononegaviral RNA-dependent RNA polymerase (RdRp) domain showing homology to the protein L of mymonaviruses, whereas the possible functions of the remaining two ORFs are still unknown. The internal cDNA sequence (10-7829) of BcMyV1 was 97.9% identical to the full-length cDNA sequence of Sclerotinia sclerotiorum negative stranded RNA virus 7 (SsNSRV7), a virus-like contig obtained from metatranscriptomes, indicating BcMyV1 should be a strain of SsNSRV7. Phylogenetic analysis based on RdRp domains showed that BcMyV1 was clustered with the viruses in the family , suggesting it is a member of . BcMyV1 may be widely distributed in regions where occurs in China and even over the world, although it infected only 0.8% of tested strains.
这里，我们描述了一种从植物病原菌中分离得到的负单链（-ss）RNA 真菌病毒，即 Botrytis cinerea mymonavirus 1（BcMyV1）。BcMyV1 的基因组长度为 7863 个核苷酸，包含三个开放阅读框（ORF1-3）。ORF1 编码一个包含保守的单负病毒 RNA 依赖的 RNA 聚合酶（RdRp）结构域的大多肽，与 mymonaviruses 的蛋白 L 具有同源性，而其余两个 ORF 的可能功能仍不清楚。BcMyV1 的内部 cDNA 序列（10-7829）与从宏转录组获得的 Sclerotinia sclerotiorum 负链 RNA 病毒 7（SsNSRV7）的全长 cDNA 序列（10-7829）具有 97.9%的同一性，这表明 BcMyV1 应该是 SsNSRV7 的一个株系。基于 RdRp 结构域的系统发育分析表明，BcMyV1 与家族中的病毒聚类在一起，这表明它是该家族的一员。BcMyV1 可能广泛分布于在中国乃至全球发生的地区，尽管它仅感染了 0.8%的测试 菌株。
