Hu Kang, Luo Qing, Zhu Xiao-Feng, Sun Su-Hong, Feng Guo-Li, Liu Zheng-Yun, Chen Xiao-Ming
Department of Thyroid Breast Surgery,Affiliated Hospital of Zunyi Medical College Zunyi 563000,China.
Cancer Research Laboratory,Affiliated Hospital of Zunyi Medical College Zunyi 563000,China.
Zhongguo Zhong Yao Za Zhi. 2019 Jul;44(13):2835-2840. doi: 10.19540/j.cnki.cjcmm.20190321.305.
SATB1 plays a crucial role in the invasion and metastasis of breast cancer,and inhibition of SATB1 expression can effectively control breast cancer metastasis. In this study,homogeneous polysaccharides were isolated from Poria cocos and their sulfated derivatives were prepared to screen out the polysaccharide compositions with inhibitory effects on SATB1 expression. Smal-molecule components were removed from P. cocos by ethanol extraction,and P. cocos crude polysaccharide PPS was obtained by water extraction and ethanol precipitation. Then PPS was successively separated by DEAE Sepharose fast flow anion-exchange and Superdex-75 gel permeation chromatographic steps to give PPSW-1. The structure of PPSW-1 was identified and its sulfated derivatives were prepared. Then their inhibitory effects on human breast cancer MDA-MB-231 cells were investigated. A kind of polysaccharide,PPSW-1 with inhibitory effect on human breast cancer MDA-MB-231 cells,was obtained from P. cocos,with a relative molecular weight of 3. 06×104,and structure of 1,6-branched 1,3-α-D-galactan. PPSW-1 and its sulfated derivative Sul-W-1 showed good inhibitory effect on cells migration,and the water solubility of Sul-W-1 was better than that of PPSW-1. In addition,it was found that polysaccharide of P. cocos and its sulfated derivative can inhibit expression of SATB1. In this study,a kind of homogeneous polysaccharide with inhibitory effect on human breast cancer MDA-MB-231 cells was isolated from P. cocos,and its sulfated derivative with similar efficacy but better solubility was prepared,laying the foundation for the substance basis study of P. cocos.
SATB1在乳腺癌的侵袭和转移中起关键作用，抑制SATB1表达可有效控制乳腺癌转移。本研究从茯苓中分离出均一多糖并制备其硫酸化衍生物，以筛选出对SATB1表达有抑制作用的多糖成分。通过乙醇提取去除茯苓中的小分子成分，再经水提取和乙醇沉淀得到茯苓粗多糖PPS。然后依次通过DEAE Sepharose fast flow阴离子交换和Superdex-75凝胶渗透色谱步骤对PPS进行分离，得到PPSW-1。鉴定了PPSW-1的结构并制备了其硫酸化衍生物。随后研究了它们对人乳腺癌MDA-MB-231细胞的抑制作用。从茯苓中获得了一种对人乳腺癌MDA-MB-231细胞有抑制作用的多糖PPSW-1，其相对分子质量为3.06×104，结构为1,6-分支的1,3-α-D-半乳聚糖。PPSW-1及其硫酸化衍生物Sul-W-1对细胞迁移显示出良好的抑制作用，且Sul-W-1的水溶性优于PPSW-1。此外，发现茯苓多糖及其硫酸化衍生物可抑制SATB1的表达。本研究从茯苓中分离出一种对人乳腺癌MDA-MB-231细胞有抑制作用的均一多糖，并制备了具有相似功效但溶解性更好的硫酸化衍生物，为茯苓的物质基础研究奠定了基础。
