Laboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Departamento de Bioquímica, Centro de Biociências, Universidade Federal do Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte, 59078-970, Brazil.
Programa de Pós-graduação em Ciências da Saúde, Universidade Federal do Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte, 59012-570, Brazil.
Mar Biotechnol (NY). 2021 Feb;23(1):77-89. doi: 10.1007/s10126-020-10004-5. Epub 2020 Nov 10.
Seaweeds are important source of bioactive compounds, including sulfated polysaccharides (SP). Because of their structural heterogeneity, these compounds are promising sources of anticancer compounds. SP from brown and red seaweeds have shown antimelanoma activity in different in vitro and in vivo models. However, SP from green seaweed are still poorly evaluated. Therefore, SP were extracted from the green alga Caulerpa cupressoides var. flabellata, and their antiproliferative, anti-migratory, and inhibitory effect on melanin production on B16-F10 melanoma cells was evaluated. Cell assays, including flow cytometry, demonstrated that SP (100-1000 μg mL) are non-cytotoxic, do not induce apoptosis or necrosis, and do not interfere with cell cycle. However, SP (1000 μg mL) were found to significantly inhibit cell colony formation (80-90%), cell migration (40-75%), and melanin production (~ 20%). In summary, these results showed that SP inhibited important melanoma development events without cytotoxicity effects, suggesting that C. cupressoides may be an important source of SP with antitumor properties.
海藻是生物活性化合物的重要来源，包括硫酸多糖（SP）。由于其结构的异质性，这些化合物是有前途的抗癌化合物来源。来自褐藻和红藻的 SP 在不同的体外和体内模型中显示出抗黑色素瘤活性。然而，来自绿藻的 SP 仍未得到充分评估。因此，从绿藻角叉菜属杯状变种中提取了 SP，并评估了它们对 B16-F10 黑色素瘤细胞的增殖、迁移和黑色素生成的抑制作用。细胞试验，包括流式细胞术，表明 SP（100-1000μg mL）是非细胞毒性的，不会诱导细胞凋亡或坏死，也不会干扰细胞周期。然而，发现 SP（1000μg mL）显著抑制细胞集落形成（80-90%）、细胞迁移（40-75%）和黑色素生成（约 20%）。总之，这些结果表明 SP 抑制了重要的黑色素瘤发展事件，而没有细胞毒性作用，提示角叉菜属可能是具有抗肿瘤特性的 SP 的重要来源。
