Ullah A H, Phillippy B Q
Southern Regional Research Laboratory ARS, USDA, New Orleans, Louisiana 70124.
Prep Biochem. 1988;18(4):483-9. doi: 10.1080/00327488808062546.
Aspergillus ficuum phytase was covalently immobilized on Fractogel TSK HW-75 containing 2-oxy-l-alkylpyridinium salts. A packed-bed bioreactor was constructed with the immobilized phytase. An HPLC ion-exchange method was used to analyze the enzymatic products of the bioreactor. Immobilized fungal phytase was able to hydrolyze myo-inositol Hexa-, penta-, tetra-, tri-, and diphosphates. When the substrate solution was recirculated for 5 hr in the bioreactor about 50% inorganic orthophosphate was released and myo-inositol-diphosphate and mono-phosphate were the only remaining products.
泡盛曲霉植酸酶被共价固定在含有2-氧代-1-烷基吡啶鎓盐的Fractogel TSK HW-75上。用固定化的植酸酶构建了填充床生物反应器。采用高效液相色谱离子交换法分析生物反应器的酶促产物。固定化真菌植酸酶能够水解肌醇六磷酸、五磷酸、四磷酸、三磷酸和二磷酸。当底物溶液在生物反应器中循环5小时时，约50%的无机正磷酸盐被释放出来，肌醇二磷酸和单磷酸是仅存的产物。
