使用针对传染性胃肠炎病毒的中和单克隆抗体在体内缺乏保护作用。
Lack of protection in vivo with neutralizing monoclonal antibodies to transmissible gastroenteritis virus.
作者信息
Wesley R D, Woods R D, Correa I, Enjuanes L
机构信息
National Animal Disease Center, USDA, Ames, IA 50010.
出版信息
Vet Microbiol. 1988 Dec;18(3-4):197-208. doi: 10.1016/0378-1135(88)90087-9.
Abstract
Monoclonal antibodies (Mabs) specific for the E1 and E2 surface glycoproteins of the transmissible gastroenteritis virus (TGEV) of swine were examined either alone or in combination to evaluate their potential value in protecting neonatal pigs against a lethal dose of TGEV. Cesarean-delivered colostrum-deprived (CDCD) piglets were given one pre-challenge dose of Mab and an equal dose of the same Mab at each successive feeding after challenge. In vivo challenge results demonstrated that neither Mabs given individually nor combinations of the Mabs were able to protect neonatal pigs against a lethal dose of TGEV. However, in parallel experiments, polyclonal antibodies from immune colostrum or serum were protective.
摘要
对猪传染性胃肠炎病毒（TGEV）的E1和E2表面糖蛋白具有特异性的单克隆抗体（Mabs），单独或联合使用，以评估它们在保护新生仔猪免受致死剂量TGEV感染方面的潜在价值。通过剖腹产获得的初乳缺乏的（CDCD）仔猪在攻毒前给予一剂Mab，并在攻毒后的每次连续喂食时给予等量的相同Mab。体内攻毒结果表明，单独给予的Mabs或Mabs的组合均不能保护新生仔猪免受致死剂量的TGEV感染。然而，在平行实验中，来自免疫初乳或血清的多克隆抗体具有保护作用。
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