Fernández Belén, Ferrer Isidro, Gil Fernando, Hilfiker Sabine
Institute of Parasitology and Biomedicine "López-Neyra", Consejo Superior de Investigaciones Científicas (CSIC), Avda del Conocimiento s/n, 18016 Granada, Spain.
Institute of Neuropathology, IDIBELL-University Hospital Bellvitge, University of Barcelona, Llobregat, Spain.
Toxicol Rep. 2017;4:188-193. doi: 10.1016/j.toxrep.2017.03.005. Epub 2017 Mar 31.
Iron levels in the healthy human brain are known to be high in certain areas such as the substantia nigra (SN), and increase further with age. In addition, there is some evidence for a further increase in iron load in the SN of Parkinson's disease (PD) patients as compared to controls, which correlates with motor disability. Here, we have analyzed total iron levels in cells as well as mouse and human brain samples by atomic absorption spectroscopy (AAS). Our data indicate that iron load is more pronounced in cells with dopaminergic features. Moreover, region-specific differences in iron load reflecting those in the human brain were detected in rodent brains as well. Whilst altered iron load was not observed in other regions also affected in PD patients, we report a significant increase in iron load in the SN of Lewy body disease patients as compared to Alzheimer's disease (AD) patients or controls, which correlates with neurodegeneration in this brain area.
已知健康人脑中某些区域（如黑质）的铁含量较高，并且会随着年龄的增长进一步增加。此外，有证据表明，与对照组相比，帕金森病（PD）患者黑质中的铁负荷进一步增加，这与运动功能障碍相关。在此，我们通过原子吸收光谱法（AAS）分析了细胞以及小鼠和人脑样本中的总铁含量。我们的数据表明，具有多巴胺能特征的细胞中铁负荷更为明显。此外，在啮齿动物脑中也检测到了反映人类大脑中铁负荷的区域特异性差异。虽然在PD患者也受影响的其他区域未观察到铁负荷改变，但我们报告，与阿尔茨海默病（AD）患者或对照组相比，路易体病患者黑质中的铁负荷显著增加，这与该脑区的神经退行性变相关。
