Department of Physiology, Shandong Provincial Key Laboratory of Pathogenesis and Prevention of Neurological Disorders and State Key Disciplines, Physiology, Medical College of Qingdao University, Qingdao, China.
Neurology. 2013 Jan 29;80(5):492-5. doi: 10.1212/WNL.0b013e31827f0ebb. Epub 2013 Jan 9.
The present study aimed to evaluate alterations in the levels of iron, divalent metal transporter 1 (DMT1) with the iron-responsive element (IRE), transferrin receptor 1 (TfR1), ferroportin 1 (FPN1), and iron regulatory protein 1 (IRP1) in the temporal cortex of human brains with Parkinson disease (PD).
Iron content was measured using an ICP-MS 7500CE detector. IRP1, DMT1+IRE, TfR1, and FPN1 expressions were determined by Western blotting.
Iron content was significantly lower in the temporal cortex of patients with PD when compared with age-matched healthy controls. Unexpectedly, the levels of DMT1+IRE, TfR1, FPN1, and IRP1 were decreased in the temporal cortex in PD brains. No changes were observed in the temporal cortex of postmortem Alzheimer disease brains.
Iron deprivation and iron-related protein dysregulation suggest that a different iron regulatory mechanism may exist, and that iron redistribution may occur between the temporal cortex and the substantia nigra of patients with PD.
本研究旨在评估帕金森病(PD)患者大脑颞叶中铁、二价金属转运蛋白 1(DMT1)与铁反应元件(IRE)、转铁蛋白受体 1(TfR1)、亚铁转运蛋白 1(FPN1)和铁调节蛋白 1(IRP1)水平的变化。
使用 ICP-MS 7500CE 检测器测量铁含量。通过 Western blot 测定 IRP1、DMT1+IRE、TfR1 和 FPN1 的表达。
与年龄匹配的健康对照组相比,PD 患者颞叶中的铁含量明显降低。出乎意料的是,PD 患者大脑颞叶中的 DMT1+IRE、TfR1、FPN1 和 IRP1 水平降低。在死后阿尔茨海默病大脑的颞叶中未观察到变化。
铁剥夺和铁相关蛋白失调表明可能存在不同的铁调节机制,并且 PD 患者的颞叶和黑质之间可能发生铁重新分布。