Soares Genaina Guimarães, Magalhães Pâmela Amorim, Fonseca Ana Beatriz Monteiro, Tostes Monica Almeida, Silva Eduardo Moreira da, Coutinho Thereza Christina Lopes
Oral Health Prev Dent. 2017;15(3):269-277. doi: 10.3290/j.ohpd.a38160.
To evaluate the effect of CPP-ACPF paste and fluoride toothpastes on enamel subjected to erosion and erosion plus abrasion in vitro.
A total of 220 human enamel blocks were divided into eleven groups (n = 20): CPP-ACPF paste (MPP), potassium nitrate/sodium fluoride toothpaste (PE), sodium fluoride toothpaste (FD), fluoride-free toothpaste (SO) and control (erosion only with no paste or toothpastes; CO) according to the experimental design: erosion or erosion plus abrasion immediately after erosion (ERO+I-ABR) or 30 min after erosion (ERO+30min-ABR). For 5 days, the specimens were subjected to: (1) erosive challenge (EC) (cola drink, 4 x 5 min/day), topical application of the undiluted paste or diluted toothpastes (1:2 w/w) (4 x 1 min/ day) plus 1 h in artificial saliva (AS) between cycles and overnight; or (2) EC plus abrasion (4 x /60 s/day) performed with the diluted toothpastes (no MMP) plus 1 h in AS between cycles and overnight. Erosion depth was quantified through a 3D profilometer. Data were analysed using Kruskal-Wallis, Mann-Whitney and Wilcoxon tests (p = 0.05).
CPP-ACPF paste and NaF toothpaste showed lowest enamel wear among groups and reduced tissue loss by 89% in erosion challenge. Abrasion led to higher enamel wear than erosion only (p = 0.030). ERO+30min-ABR had no protective effect when compared to ERO+I-ABR (p > 0.05).
A high frequency of CPP-ACPF paste application (4x daily) is effective in reducing the effects of erosion. A waiting period before performing toothbrushing does not protect enamel against erosion regardless the composition of the toothpastes.
评估酪蛋白磷酸肽-无定形磷酸钙氟化物（CPP-ACPF）糊剂和含氟牙膏对体外遭受侵蚀以及侵蚀加磨损的牙釉质的影响。
根据实验设计，将总共220个人类牙釉质块分为11组（n = 20）：CPP-ACPF糊剂（MPP）、硝酸钾/氟化钠牙膏（PE）、氟化钠牙膏（FD）、无氟牙膏（SO）以及对照组（仅侵蚀，不使用任何糊剂或牙膏；CO）：侵蚀后立即进行侵蚀或侵蚀加磨损（ERO+I-ABR）或侵蚀后30分钟进行侵蚀或侵蚀加磨损（ERO+30分钟-ABR）。持续5天，对标本进行以下处理：（1）侵蚀挑战（EC）（可乐饮料，每天4次，每次5分钟），局部涂抹未稀释的糊剂或稀释后的牙膏（1:2重量/重量）（每天4次，每次1分钟），并且在各周期之间以及过夜时在人工唾液（AS）中浸泡1小时；或者（2）EC加磨损（每天4次，每次60秒），使用稀释后的牙膏进行（不使用MMP），并且在各周期之间以及过夜时在AS中浸泡1小时。通过三维轮廓仪对侵蚀深度进行量化。使用Kruskal-Wallis检验、Mann-Whitney检验和Wilcoxon检验分析数据（p = 0.05）。
CPP-ACPF糊剂和氟化钠牙膏在各实验组中牙釉质磨损最低，在侵蚀挑战中组织损失减少了89%。磨损导致的牙釉质磨损高于仅侵蚀（p = 0.030）。与ERO+I-ABR相比，ERO+30分钟-ABR没有保护作用（p>0.05）。
高频率应用CPP-ACPF糊剂（每天4次）可有效减少侵蚀的影响。无论牙膏成分如何，刷牙前的等待期都不能保护牙釉质免受侵蚀。
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