Elloumi Nesrine, Fakhfakh Raouia, Ayadi Lobna, Sellami Khadija, Abida Olfa, Ben Jmaa Mariem, Sellami Tahya, Kammoun Khawla, Masmoudi Hatem
Immunology Department, Habib Bourguiba Hospital (NE, RF, OA, MBJ, HM), University of Sfax, Sfax, Tunisia.
Anatomopathology Department, Habib Bourguiba Hospital (LA, TS), University of Sfax, Sfax, Tunisia.
J Histochem Cytochem. 2017 Jul;65(7):389-398. doi: 10.1369/0022155417709234. Epub 2017 May 22.
Toll-like receptor 4 (TLR-4), a bacterial lipopolysaccharide sensor, is an innate immunity essential modulator. It is expressed on both immune and non-immune cells and may contribute to the cutaneous and renal manifestations during lupus erythematosus (LE). Our purpose is to evaluate TLR-4 expression and analyzing its role in lupus nephritis (LN) and chronic cutaneous lupus erythematosus (CLE) pathogenesis. TLR-4 immunohistochemical staining was performed on 30 LN renal biopsies compared with 11 healthy renal tissues and 30 skin biopsies from CLE patients compared with 15 normal individuals. CLE patients' biopsies showed a strong and diffuse TLR-4 expression throughout the epidermis and labeled inflammatory infiltrate and glands in the dermis whereas controls' skin expressed weakly TLR-4 only in the epidermis basal layer. LN glomeruli and tubules showed an increased and more intense TLR-4 expression compared with normal controls where TLR-4 expression was weak and rarely detected in glomeruli, diffuse and weak in tubules. A significant difference in TLR-4 expression between LN classes, both in glomeruli and tubules, was observed. These data confirm an up-regulation of TLR-4 expression in the affected tissues of CLE and LN patients and highlight the critical role of TLR-4 in the pathogenesis of cutaneous and renal disorders in LE.
Toll样受体4（TLR-4）作为一种细菌脂多糖传感器，是先天性免疫的重要调节因子。它在免疫细胞和非免疫细胞上均有表达，可能与红斑狼疮（LE）的皮肤和肾脏表现有关。我们的目的是评估TLR-4的表达，并分析其在狼疮性肾炎（LN）和慢性皮肤型红斑狼疮（CLE）发病机制中的作用。对30例LN肾活检组织进行TLR-4免疫组化染色，并与11例健康肾组织进行比较；对30例CLE患者的皮肤活检组织进行TLR-4免疫组化染色，并与15例正常个体进行比较。CLE患者的活检组织显示，整个表皮均有强烈而弥漫的TLR-4表达，真皮中的炎性浸润和腺体也有标记，而对照组皮肤仅在表皮基底层有微弱的TLR-4表达。与正常对照组相比，LN的肾小球和肾小管显示出TLR-4表达增加且更强，正常对照组中TLR-4在肾小球中表达微弱且很少检测到，在肾小管中呈弥漫性且微弱。观察到LN各类型之间，无论是在肾小球还是肾小管中，TLR-4表达均存在显著差异。这些数据证实了CLE和LN患者受累组织中TLR-4表达上调，并突出了TLR-4在LE皮肤和肾脏疾病发病机制中的关键作用。
