Li Kun, Luo Houqiang, Zhang Hui, Mehmood Khalid, Shahzad Muhammad, Zhang Lihong, Li Jiakui
a College of Veterinary Medicine , Huazhong Agricultural University , Wuhan , People's Republic of China.
b University College of Veterinary & Animal Sciences, The Islamia University of Bahawalpur , Bahawalpur , Pakistan.
Mitochondrial DNA A DNA Mapp Seq Anal. 2018 May;29(4):624-628. doi: 10.1080/24701394.2017.1331348. Epub 2017 May 21.
Ascaris suum (A. suum) is the most commonly occurring worldwide internal parasite of pigs; however, little is known about this organism in Tibetan pigs in China. A study was carried to isolate and identify the characteristics of internal transcribed spacer region (ITS) gene of A. suum derived from Tibetan pigs. Adult nematodes were collected from Tibetan pigs in 2015-2016. Total genomic DNA of the extracted parasites was performed and a fragment of the ITS of mitochondrial (mt) gene was amplified. The amplicons were cloned into PGEM®-T Easy Vector (Promega, WI) and the positive clones were sequenced by ABI 3730 × 1 sequencer. The sequence and phylogenetic analysis were performed by ClustalWVer. 1.4 and MEGA 6.0 software, respectively. Results indicated that the identity of A. suum isolates was 98.4%-99.9% with previously reported pig isolates, and 99.4%-99.7% with A. lumbricoides isolates. To the best of our knowledge, this is the first report describing the characteristics of ITS gene of A. suum derived from the Tibetan pigs from high and remote areas depicting high identity with the isolates of both A. suum and A. lumbricoides.
猪蛔虫（Ascaris suum）是全球猪最常见的体内寄生虫；然而，在中国藏猪中对这种生物体的了解甚少。开展了一项研究，以分离和鉴定源自藏猪的猪蛔虫内部转录间隔区（ITS）基因的特征。2015年至2016年从藏猪中收集成虫线虫。对提取的寄生虫的总基因组DNA进行操作，并扩增线粒体（mt）基因ITS的一个片段。将扩增子克隆到PGEM®-T Easy载体（Promega，威斯康星州）中，并通过ABI 3730×1测序仪对阳性克隆进行测序。分别使用ClustalWVer. 1.4和MEGA 6.0软件进行序列和系统发育分析。结果表明，猪蛔虫分离株与先前报道的猪分离株的同一性为98.4%-99.9%，与蛔虫分离株的同一性为99.4%-99.7%。据我们所知，这是第一份描述源自高海拔偏远地区藏猪的猪蛔虫ITS基因特征的报告，该基因与猪蛔虫和蛔虫分离株具有高度同一性。
