Alizadeh Taher, Ganjali Mohammad Reza, Rafiei Faride, Akhoundian Maedeh
Department of Analytical Chemistry, Faculty of Chemistry, University College of Science, University of Tehran, P.O. Box 14155-6455, Tehran, Iran; Endocrinology & Metabolism Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Department of Analytical Chemistry, Faculty of Chemistry, University College of Science, University of Tehran, P.O. Box 14155-6455, Tehran, Iran.
Mater Sci Eng C Mater Biol Appl. 2017 Aug 1;77:300-307. doi: 10.1016/j.msec.2017.03.168. Epub 2017 Mar 27.
A novel timolol voltammetric sensor based on the nano-sized molecularly imprinted polymer (nano-MIP)-modified carbon paste electrode was introduced. Timolol-imprinted polymers (MIP) were synthesized by the ultrasonic assisted suspension polymerization in silicon oil. The MIP nanoparticles were then embedded in a carbon paste (CP) electrode in order to prepare the nano-MIP-CP electrode. Timolol was extracted in the electrode for a definite time and then it was analyzed by square wave voltammetry, found to be an effective determination method. The electrode showed higher response to timolol, compared to the CP electrode, and CP electrode modified with non-imprinted polymer (nano-NIP-CP). Various factors, known to affect the response behavior of the nano-MIP-CP electrode, were investigated and optimized. The sensor exhibited distinct linear response ranges of 1.0×10-2.1×10M with the sensitivity of 71.523μAμM. The lower detection limit of the sensor was calculated to be 2.3×10M (S/N=3). The sensor was applied successfully for timolol determination in pharmaceutical formulations, blood serum and urine samples.
介绍了一种基于纳米尺寸分子印迹聚合物（nano-MIP）修饰碳糊电极的新型噻吗洛尔伏安传感器。噻吗洛尔印迹聚合物（MIP）通过在硅油中超声辅助悬浮聚合合成。然后将MIP纳米颗粒嵌入碳糊（CP）电极中，以制备纳米MIP-CP电极。将噻吗洛尔在电极中萃取一定时间，然后通过方波伏安法进行分析，发现这是一种有效的测定方法。与CP电极以及用非印迹聚合物修饰的CP电极（纳米NIP-CP）相比，该电极对噻吗洛尔表现出更高的响应。研究并优化了已知会影响纳米MIP-CP电极响应行为的各种因素。该传感器呈现出1.0×10 - 2.1×10M的明显线性响应范围，灵敏度为71.523μA/μM。计算得出该传感器的下限检测限为2.3×10M（S/N = 3）。该传感器已成功应用于药物制剂、血清和尿液样本中噻吗洛尔的测定。
