Dick T A, Dos Santos L A
Departamento de Engenharia de Minas, Materiais e Metalurgia, Universidade Federal do Rio Grande do Sul, 90650-001 Porto Alegre, RS, Brazil.
Mater Sci Eng C Mater Biol Appl. 2017 Aug 1;77:874-882. doi: 10.1016/j.msec.2017.03.301. Epub 2017 Apr 4.
In this work, a biomimetic synthesis reaction for the production of hydroxyapatite (HA)/natural rubber (NR) composites is presented. HA was synthesized in the presence of solubilized NR in tetrahydrofuran (THF), which permits that negatively charged domains in proteins and lipids in NR work as nucleation sites for hydroxyapatite. The mechanical and physicochemical properties of composites containing 10, 20 and 30wt% HA were studied. NR influenced HA crystallite morphology, shape and size and was able to disperse the HA particles in THF. The dispersion of HA in the composites promoted the enhancement of mechanical properties and loss of solubility of composites, which is believed to be macroscopic manifestations of the physical adsorption of NR to HA particles, known as the bound rubber phenomenon. Preliminary cell studies indicated that HepG2 cells presented viabilities ranging from 71.6 to 91.3%, with cell proliferation positively influenced by HA content at shorter culture times. The good dispersion of HA in the composites is expected to improve NR bioactivity and direct its use towards bone applications.
在这项工作中，提出了一种用于制备羟基磷灰石（HA）/天然橡胶（NR）复合材料的仿生合成反应。HA是在溶解于四氢呋喃（THF）的NR存在下合成的，这使得NR中蛋白质和脂质的带负电区域能够作为羟基磷灰石的成核位点。研究了含有10%、20%和30%（重量）HA的复合材料的力学和物理化学性质。NR影响了HA微晶的形态、形状和尺寸，并能够在THF中分散HA颗粒。HA在复合材料中的分散促进了复合材料力学性能的提高和溶解性的降低，这被认为是NR对HA颗粒物理吸附的宏观表现，即结合橡胶现象。初步细胞研究表明，HepG2细胞的活力在71.6%至91.3%之间，在较短培养时间内，细胞增殖受到HA含量的积极影响。预计HA在复合材料中的良好分散将提高NR的生物活性，并指导其在骨应用中的使用。
