Ye Yong, Liu Kun, Zeng Qinghua, Zeng Qingmei
School of Food Science and Engineering, Hefei University of Technology, 124 ShenHua Building, 193rd TunXi Road, Hefei, 230009, Anhui, China.
AMB Express. 2017 Dec;7(1):99. doi: 10.1186/s13568-017-0402-5. Epub 2017 May 19.
To test the antimicrobial activity of different extracts and fermentation broth from puffball(Bovistella radicata), the different extracts and fermentation broth of puffball were prepared, the active fraction was investigated by UPLC-UV-MS and semi-preparative chromatograph.
Through zones of inhibition (ZOI) and minimum inhibitory concentrations (MIC) tests, the supernatant of fermentation possessed best antimicrobial activity in all extracts whose MIC value is 31.2 μg/ml against T. rubrum, T. mentagrophytes, S. aureus and P. aeruginosa. And ZOI value is 29.01, 21.02, 35.02, 28.01 mm against T. rubrum, T. mentagrophytes, S. aureus and P. aeruginosa. Then we compare the puffball fermentation supernatant with blank contrast by LC-MS. There are the characteristic peaks named PBR-1 and PBR-2 with the puffball fermentation supernatant, the separation of compound PBR-1 and PBR-2 was done on semi-preparative C18 column and the MIC and ZOI of compound PBR-1 and PBR-2 are 15.6 μg/ml and 34 mm with the antifungal test.
The fermentation supernatant and compound PBR-1 and PBR-2 have promising antifungal activity against T. rubrum and T. mentagrophytes.
为测试马勃（秃马勃）不同提取物及发酵液的抗菌活性，制备了马勃的不同提取物及发酵液，采用超高效液相色谱 - 紫外 - 质谱联用仪（UPLC - UV - MS）和半制备色谱对活性成分进行研究。
通过抑菌圈（ZOI）和最低抑菌浓度（MIC）测试，发酵上清液在所有提取物中抗菌活性最佳，其对红色毛癣菌、须癣毛癣菌、金黄色葡萄球菌和铜绿假单胞菌的MIC值为31.2 μg/ml。对红色毛癣菌、须癣毛癣菌、金黄色葡萄球菌和铜绿假单胞菌的抑菌圈直径分别为29.01、21.02、35.02、28.01 mm。然后通过液相色谱 - 质谱联用仪（LC - MS）将马勃发酵上清液与空白对照进行比较。马勃发酵上清液中有特征峰PBR - 1和PBR - 2，在半制备C18柱上对化合物PBR - 1和PBR - 2进行分离，经抗真菌测试，化合物PBR - 1和PBR - 2的MIC值为15.6 μg/ml，抑菌圈直径为34 mm。
发酵上清液以及化合物PBR - 1和PBR - 2对红色毛癣菌和须癣毛癣菌具有良好的抗真菌活性。
