Nongonierma A B, Hennemann M, Paolella S, FitzGerald R J
Department of Biological Sciences, University of Limerick, Limerick, Ireland.
Food Funct. 2017 Jun 21;8(6):2249-2257. doi: 10.1039/c7fo00165g.
Wheat gluten, a Pro-rich dietary protein, was investigated for its potential to produce dipeptidyl peptidase IV (DPP-IV) inhibitory peptides during enzymatic hydrolysis with Debitrase HYW20. Nine gluten hydrolysates (H1-H9) were generated using a 2 factor × 3 level design of experiments (DOE) including the incubation temperature (40, 50 and 60 °C) and the enzyme: substrate ratio (E : S, 0.5, 1.0 and 1.5% (w/w)). Their DPP-IV half maximal inhibitory concentration (IC) ranged from 0.24 ± 0.02 (H9) to 0.66 ± 0.06 mg mL (H2A and H7) and their degree of hydrolysis (DH) from 31.7 ± 0.9 (H7) to 62.2 ± 3.0% (H6). Gluten and H9, the most potent DPP-IV inhibitory hydrolysate, were subjected to simulated gastrointestinal digestion (SGID), yielding Gluten_CorPP and H9_CorPP, respectively. H9_CorPP had a higher DPP-IV inhibitory potency than Gluten_CorPP (i.e., DPP-IV IC values of 0.33 ± 0.03 vs. 1.45 ± 0.26 mg mL, respectively). H9 and H9_CorPP both contained relatively potent DPP-IV inhibitory peptides such as Val-Pro-Leu, Trp-Leu and Trp-Pro which were identified by liquid chromatography tandem mass spectrometry (LC-MS/MS). In addition, several sequences possessing features of DPP-IV inhibitory peptides, mostly consisting of a penultimate or C-terminal Pro, were identified within H9. The presence of Pro-containing peptides within H9 may contribute to its stability to digestive enzymes. Gluten hydrolysates may have antidiabetic potential for humans.
小麦面筋是一种富含脯氨酸的膳食蛋白质，研究了其在使用Debitrase HYW20进行酶水解过程中产生二肽基肽酶IV（DPP-IV）抑制肽的潜力。使用2因素×3水平的实验设计（DOE）生成了9种面筋水解产物（H1-H9），包括孵育温度（40、50和60°C）和酶：底物比（E : S，0.5、1.0和1.5%（w/w））。它们的DPP-IV半数最大抑制浓度（IC）范围为0.24±0.02（H9）至0.66±0.06 mg/mL（H2A和H7），水解度（DH）范围为31.7±0.9（H7）至62.2±3.0%（H6）。面筋和最有效的DPP-IV抑制水解产物H9进行了模拟胃肠道消化（SGID），分别产生了Gluten_CorPP和H9_CorPP。H9_CorPP比Gluten_CorPP具有更高的DPP-IV抑制效力（即DPP-IV IC值分别为0.33±0.03和1.45±0.26 mg/mL）。H9和H9_CorPP都含有相对有效的DPP-IV抑制肽，如Val-Pro-Leu、Trp-Leu和Trp-Pro，这些肽通过液相色谱串联质谱（LC-MS/MS）鉴定。此外，在H9中鉴定出了几个具有DPP-IV抑制肽特征的序列，大多由倒数第二个或C末端脯氨酸组成。H9中含脯氨酸肽的存在可能有助于其对消化酶的稳定性。面筋水解产物可能对人类具有抗糖尿病潜力。
