Department of Biological Sciences, University of Limerick, Limerick, Ireland.
Molecules. 2023 Jan 17;28(3):915. doi: 10.3390/molecules28030915.
The proteins from two conventionally (CC1 and CC2) and one organically cultivated (OC) hempseed samples were extracted (by alkaline solubilization followed by isoelectric precipitation) and compared in terms of their physicochemical, digestibility and bioactivity properties. The OC hempseed had higher total protein and lower nonprotein nitrogen content. Protein extracts showed bimodal particle size distributions, with OC showing the smallest and CC1 the largest mean particle diameter (d(0.5)), i.e., 89.0 and 120.0 µm, respectively. Chromatographic analysis showed similar protein profiles for all three protein extracts. The protein extracts were subjected to simulated gastrointestinal digestion (SGID). Degree of hydrolysis (DH) measurement showed that the highest extent of digestion upon SGID was associated with CC1 (11.0 ± 1.5%), which also had the lowest antioxidant activity. Only the OC and OC digested samples had lipase inhibitory activity. The results indicate that the cultivation method impacted the composition, physicochemical, digestibility, and biofunctional properties of hempseed proteins.
从两种传统（CC1 和 CC2）和一种有机种植（OC）的大麻种子样品中提取蛋白质（通过碱性溶解后等电沉淀），并在理化性质、消化率和生物活性方面进行比较。OC 大麻种子的总蛋白含量较高，而非蛋白氮含量较低。蛋白质提取物的粒径分布呈双峰分布，OC 的平均粒径（d(0.5)）最小，为 89.0 µm，而 CC1 的最大，为 120.0 µm。色谱分析显示，三种蛋白质提取物的蛋白图谱相似。对蛋白质提取物进行模拟胃肠道消化（SGID）。水解度（DH）测量表明，SGID 时消化程度最高的是 CC1（11.0±1.5%），其抗氧化活性也最低。只有 OC 和 OC 消化样品具有脂肪酶抑制活性。结果表明，种植方式影响了大麻种子蛋白的组成、理化性质、消化率和生物功能特性。
