Centre for Biomedical Technology, Optics, Photonics and Biophotinics Laboratory, Campus Montegancedo, Universidad Politécnica de Madrid, 28223 Madrid, Spain.
BioOptical Detection, Centro de Empresas, Campus Montegancedo, 28223 Madrid, Spain.
Sensors (Basel). 2017 May 19;17(5):1158. doi: 10.3390/s17051158.
A novel compact optical biochip based on a thin layer-sensing surface of nitrocellulose is used for in-situ label-free detection of metalloproteinase (MMP9) related to dry eye disease. In this article, a new integrated chip with different interferometric transducers layout with an optimal sensing surface is reported for the first time. We demonstrate that specific antibodies can be immobilized onto these transducers with a very low volume of sample and with good orientation. Many sensing transducers constitute the presented biochip in order to yield statistical data and stability in the acquired measurements. As a result, we report the recognition curve for pure recombinant MMP9, tests of model tears with MMP9, and real tear performance from patients, with a promising limit of detection.
一种基于硝化纤维素薄层传感面的新型紧凑型光学生物芯片，用于原位无标记检测与干眼症相关的金属蛋白酶（MMP9）。本文首次报道了一种具有不同干涉传感器布局的新型集成芯片，具有最佳的传感面。我们证明，这些传感器可以用非常少量的样品和良好的定向来固定特定的抗体。许多传感传感器构成了所提出的生物芯片，以产生在获得的测量中数据的统计和稳定性。结果，我们报告了纯重组 MMP9 的识别曲线、含有 MMP9 的模型眼泪测试以及来自患者的真实眼泪性能，具有有前途的检测限。
