Center for Biomedical Technology, Optics, Photonics and Biophotonics Lab., Universidad Politécnica de Madrid, Campus Montegancedo, Pozuelo de Alarcón, 28223 Madrid, Spain.
Department of Applied Physics and Materials Engineering, Escuela Técnica Superior de Ingenieros Industriales, Universidad Politécnica de Madrid, C/José Gutierrez Abascal 2, 28006 Madrid, Spain.
Sensors (Basel). 2018 Aug 15;18(8):2686. doi: 10.3390/s18082686.
Food allergy is a common disease worldwide with over 6% of the population (200⁻250 million people) suffering from any food allergy nowadays. The most dramatic increase seems to be happening in children and young people. Therefore, improvements in the diagnosis efficiency of these diseases are needed. Immunoglobulin type E (IgE) biomarker determination in human serum is a typical in vitro test for allergy identification. In this work, we used a novel biosensor based on label-free photonic transducers called BICELLs (Biophotonic Sensing Cells) for IgE detection. These BICELLs have a thin film of nitrocellulose over the sensing surface, they can be vertical optically interrogated, and are suitable for being integrated on a chip. The BICELLs sensing surface sizes used were 100 and 800 µm in diameter. We obtained calibration curves with IgE standards by immobilizating anti-IgE antibodies and identified with standard IgE calibrators in minute sample amounts (3 µL). The results, in similar assay format, were compared with commercially available ImmunoCAP. The versatility of the interferometric nitrocellulose-based sensing surface was demonstrated since the limit of detections for BICELLs and ImmunoCAP were 0.7 and 0.35 kU/L, respectively.
食物过敏是一种全球性的常见疾病，目前全球有超过 6%的人口（2 亿至 2.5 亿人）患有任何食物过敏症。在儿童和年轻人中，这种疾病的发病率似乎增长得最为明显。因此，需要提高这些疾病的诊断效率。人血清中免疫球蛋白 E（IgE）生物标志物的测定是用于过敏鉴定的典型体外测试。在这项工作中，我们使用了一种新型的基于无标记光学生物传感器的生物传感器，称为 BICELLs（生物光子传感细胞），用于 IgE 检测。这些 BICELLs 在传感表面上有一层硝化纤维素薄膜，它们可以进行垂直光学检测，并且适合集成在芯片上。使用的 BICELLs 传感表面直径分别为 100 和 800 µm。我们通过固定抗 IgE 抗体获得了 IgE 标准品的校准曲线，并使用标准 IgE 校准器在少量样本（3 µL）中进行了鉴定。在类似的检测模式下，将结果与市售的 ImmunoCAP 进行了比较。基于干涉的硝化纤维素传感表面的多功能性得到了证明，因为 BICELLs 和 ImmunoCAP 的检测限分别为 0.7 和 0.35 kU/L。
