Liu Wenzhong, Wang Shoujian, Soetikno Brian, Yi Ji, Zhang Kevin, Chen Siyu, Linsenmeier Robert A, Sorenson Christine M, Sheibani Nader, Zhang Hao F
Department of Biomedical Engineering, Northwestern University, Evanston, Illinois, United States.
Departments of Ophthalmology and Visual Science, University of Wisconsin, Madison, Wisconsin, United States.
Invest Ophthalmol Vis Sci. 2017 Feb 1;58(2):981-989. doi: 10.1167/iovs.16-20600.
To investigate inner retinal oxygen metabolic rate (IRMRO2) during early stages of type 1 diabetes in a transgenic mouse model.
In current study, we involved seven diabetic mice (Akita/+, TSP1-/-) and seven control mice (TSP1-/-), and applied visible-light optical coherence tomography (vis-OCT) to image functional parameters including retinal blood flow rate, oxygen saturation (sO2) and the IRMRO2 value longitudinally from 5 weeks of age to 13 weeks of age. After imaging at 13 weeks of age, we analyzed the imaging results, and examined histology of mouse retina.
Between diabetic mice and the control group, we observed significant differences in venous sO2 from 9 weeks of age (P = 0.006), and significant increment in IRMRO2 from 11 weeks of age (P = 0.001) in diabetic mice compared with control group. We did not find significant differences in retinal blood flow rate as well as arterial sO2 during imaging between diabetic and control mice. Histologic examination of diabetic and control mice at 13 weeks of age also revealed no anatomical retinal alternations.
In diabetic retinopathy, complications in retinal oxygen metabolism may occur before changes of retinal anatomical structure.
在转基因小鼠模型中研究1型糖尿病早期阶段的视网膜内层氧代谢率（IRMRO2）。
在本研究中，我们纳入了7只糖尿病小鼠（秋田犬/+，TSP1-/-）和7只对照小鼠（TSP1-/-），并应用可见光光学相干断层扫描（vis-OCT）从5周龄至13周龄纵向成像包括视网膜血流速度、氧饱和度（sO2）和IRMRO2值在内的功能参数。在13周龄成像后，我们分析了成像结果，并检查了小鼠视网膜的组织学。
在糖尿病小鼠和对照组之间，我们观察到从9周龄起静脉sO2存在显著差异（P = 0.006），与对照组相比，糖尿病小鼠从11周龄起IRMRO2显著增加（P = 0.001）。在成像过程中，糖尿病小鼠和对照小鼠之间的视网膜血流速度以及动脉sO2没有显著差异。13周龄时糖尿病小鼠和对照小鼠的组织学检查也未发现视网膜解剖结构改变。
在糖尿病视网膜病变中，视网膜氧代谢并发症可能在视网膜解剖结构改变之前就已发生。
