Yu Yanming, Zhou Junde, Gong Chen, Long Zhiping, Tian Jingshen, Zhu Lin, Li Jing, Yu Hongyuan, Wang Fan, Zhao Yashuang
Department of Epidemiology, School of Public Health, Harbin Medical University, Harbin, Heilongjiang Province, P. R. China.
Department of Colorectal Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, P. R. China.
Oncotarget. 2017 Jul 18;8(29):47379-47388. doi: 10.18632/oncotarget.17649.
Long-term dietary intake influences the structure and activity of microorganisms residing in the human gut. The immune response and gut microbiota have a mutual influence on the risk of colorectal cancer (CRC). This study examines the association of gut microbiota-related dietary factors and polymorphisms in the microRNA-binding site of the interleukin 13 gene (IL13) with the risk and prognosis of CRC. Three polymorphisms (rs847, rs848, and rs1295685) were selected for genotyping in a case-control study (513 cases, 572 controls), and 386 CRC patients were followed up. Two dietary factors closely related with gut microbiota (allium vegetables, overnight meal) were significantly associated with CRC development. Although the three SNPs showed no statistically significant associations with the risk and prognosis of CRC, a significant antagonistic interaction was found between rs848 (G-T) and allium vegetable intake (ORi (odds ratio of interaction), 0.92; 95% CI (confidence interval): 0.86, 0.99; P = 0.03); moreover, significant combined and synergistic interactions were observed for all three SNPs and overnight meal intake. This is the first report of significant combined and interactive effects between dietary factors and polymorphisms in the microRNA binding site of IL13 in CRC and may provide direct guidance on intake of allium vegetable and overnight meals for individuals with specific genetic variants of IL13 to modify their susceptibility to CRC.
长期饮食摄入会影响人类肠道中微生物的结构和活性。免疫反应和肠道微生物群对结直肠癌（CRC）的风险具有相互影响。本研究探讨了与肠道微生物群相关的饮食因素以及白细胞介素13基因（IL13）的微小RNA结合位点多态性与CRC风险及预后的关联。在一项病例对照研究（513例病例，572例对照）中选择了三个多态性位点（rs847、rs848和rs1295685）进行基因分型，并对386例CRC患者进行了随访。与肠道微生物群密切相关的两个饮食因素（葱属蔬菜、隔夜餐）与CRC的发生显著相关。虽然这三个单核苷酸多态性（SNP）与CRC的风险和预后无统计学显著关联，但发现rs848（G-T）与葱属蔬菜摄入量之间存在显著的拮抗相互作用（交互作用比值比（ORi），0.92；95%置信区间（CI）：0.86，0.99；P = 0.03）；此外，观察到所有三个SNP与隔夜餐摄入量之间存在显著的联合和协同相互作用。这是关于饮食因素与IL13微小RNA结合位点多态性在CRC中存在显著联合和交互作用的首次报道，可能为具有特定IL13基因变异的个体摄入葱属蔬菜和隔夜餐以改变其对CRC的易感性提供直接指导。
