Sahub Chonticha, Tumcharern Gamolwan, Chirawatkul Prae, Ruangpornvisuti Vithaya, Ekgasit Sanong, Wanichweacharungruang Supasorn, Tuntulani Thawatchai, Palaga Tanapat, Tomapatanaget Boosayarat
Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
National Nanotechnology Center, National Science and Technology Development Agency, Pathumthani 12120, Thailand.
Colloids Surf B Biointerfaces. 2017 Aug 1;156:254-261. doi: 10.1016/j.colsurfb.2017.05.026. Epub 2017 May 13.
At present, strategies to disperse hydrophobic molecules in water without altering their chemical structures include conventional surfactant-based micellar and vesicular systems, encapsulation into water dispersible polymeric nanoparticles, and loading onto the surface of various metal nanoparticles. Here, we report a simple and low cost platform to incorporate hydrophobic molecules into a stable water dispersible nanostructure that can significantly increase the stability of the encapsulated materials. The platform is based on the incorporation of hydrophobic molecules into the self-assembled complex of gadolinium ion (Gd), sodium dodecyl sulfate (SDS), and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) called GdSH. After being incorporated, the two model hydrophobic dyes, curcumin and curcumin borondifluoride show approximately 50% and 30% improved stability, respectively. Investigation of the self-assembled 10-14 multilayered 60nm spheres with inter-layer distances of 4.25nm indicates coordination of SDS and HEPES with Gd. Incorporation of the hydrophobic molecules into the multilayered spheres results in reduction of the interlayer distance of the multilayer spheres to 4.17nm, suggesting enhanced packing of the hydrophobic chain of SDS and HEPES around the Gd. The incorporation of the two curcuminoids into the self-assembled complex also causes an increase in fluorescence quantum yield of the two dyes, thus suggesting spatial confinement of the packed dye molecules. The better cellular uptake of the nanoparticles is responsible for the expected enhancement in fluorescence image of the encapsulated materials.
目前，在不改变疏水分子化学结构的情况下将其分散于水中的策略包括基于传统表面活性剂的胶束和囊泡体系、封装于水分散性聚合物纳米颗粒中以及负载于各种金属纳米颗粒表面。在此，我们报道了一个简单且低成本的平台，可将疏水分子纳入稳定的水分散性纳米结构中，这能显著提高被封装材料的稳定性。该平台基于将疏水分子纳入钆离子（Gd）、十二烷基硫酸钠（SDS）和4-(2-羟乙基)-1-哌嗪乙磺酸（HEPES）的自组装复合物（称为GdSH）中。纳入后，两种模型疏水染料姜黄素和姜黄素硼二氟化物的稳定性分别提高了约50%和30%。对层间距为4.25nm的自组装10 - 14层60nm球体的研究表明SDS和HEPES与Gd发生了配位。将疏水分子纳入多层球体导致多层球体的层间距减小至4.17nm，这表明SDS和HEPES的疏水链在Gd周围的堆积增强。将两种姜黄素类化合物纳入自组装复合物还会导致这两种染料的荧光量子产率增加，因此表明堆积的染料分子存在空间限制。纳米颗粒更好的细胞摄取导致了被封装材料荧光图像的预期增强。
