de Almeida João N, Favero Gimenes Viviane M, Francisco Elaine C, Machado Siqueira Lumena P, Gonçalves de Almeida Renato K, Guitard Juliette, Hennequin Christophe, Colombo Arnaldo L, Benard Gil, Rossi Flavia
Central Laboratory Division (LIM03), Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil
Laboratory of Mycology (LIM 53), Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil.
J Clin Microbiol. 2017 Aug;55(8):2439-2444. doi: 10.1128/JCM.00461-17. Epub 2017 May 24.
species are relevant etiologic agents of hospital-acquired infections. High mortality rates are attributed to deep-seated infections in immunocompromised individuals, making fast and accurate species identification relevant for hastening the discovery of best-targeted therapy. Recently, taxonomy has been reassessed, and three genera have been proposed for the pathogenic species: , , and Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has replaced old phenotypic methods for microorganism identification in clinical laboratories, but spectral profile databases have to be evaluated and improved for optimal species identification performance. Vitek MS (bioMérieux) is one of the commercially available MALDI-TOF MS platforms for pathogen identification, and its spectral profile databases remain poorly evaluated for , , and species identification. We herein evaluated and improved Vitek MS for the identification of the main clinical relevant species of , , and using a large set of strains and isolates belonging to different yeast collections in Brazil and France.
某些菌种是医院获得性感染的相关病原体。高死亡率归因于免疫功能低下个体的深部感染，因此快速准确的菌种鉴定对于加快发现最佳靶向治疗方法至关重要。最近，分类学已被重新评估，并且已为致病菌种提出了三个属：、和。基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）已取代临床实验室中用于微生物鉴定的旧表型方法，但光谱图谱数据库必须进行评估和改进，以实现最佳的菌种鉴定性能。Vitek MS（生物梅里埃公司）是一种用于病原体鉴定的商用MALDI-TOF MS平台之一，其光谱图谱数据库在、和菌种鉴定方面的评估仍然很差。我们在此使用来自巴西和法国不同酵母菌种库的大量菌株和分离株，对Vitek MS进行了评估和改进，以鉴定、和的主要临床相关菌种。
