Evaluating and Improving Vitek MS for Identification of Clinically Relevant Species of Trichosporon and the Closely Related Genera Cutaneotrichosporon and Apiotrichum.

species are relevant etiologic agents of hospital-acquired infections. High mortality rates are attributed to deep-seated infections in immunocompromised individuals, making fast and accurate species identification relevant for hastening the discovery of best-targeted therapy. Recently, taxonomy has been reassessed, and three genera have been proposed for the pathogenic species: , , and Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has replaced old phenotypic methods for microorganism identification in clinical laboratories, but spectral profile databases have to be evaluated and improved for optimal species identification performance. Vitek MS (bioMérieux) is one of the commercially available MALDI-TOF MS platforms for pathogen identification, and its spectral profile databases remain poorly evaluated for , , and species identification. We herein evaluated and improved Vitek MS for the identification of the main clinical relevant species of , , and using a large set of strains and isolates belonging to different yeast collections in Brazil and France.