Goldenberger Daniel, Hinić Vladimira, Prince Spasenija Savic, Tamm Michael, Balestra Anna-Maria, Hohler Doris, Frei Reno
Division of Clinical Microbiology, University Hospital Basel, Petersgraben 4, CH-4031, Basel, Switzerland.
Division of Pathology, University Hospital Basel, Schönbeinstrasse 40, CH-4031, Basel, Switzerland.
BMC Infect Dis. 2016 Oct 25;16(1):601. doi: 10.1186/s12879-016-1910-7.
Trichosporon mycotoxinivorans is a recently described yeast-like fungal organism and its association as a pathogen for patients with cystic fibrosis (CF) was reported previously. We show the clinical course of a CF patient over 9 years as well as the applications of modern molecular and proteomic identification techniques of this rare fungus.
We present the case of a 32-year-old male CF patient with sputum cultures continuously positive with the anamorphic yeast T. mycotoxinivorans during 9 years. Furthermore, susceptibility testing of T. mycotoxinivorans to different antifungals were performed. In addition, a rapid identification method of this novel fungal pathogen with matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was applied using a simple extraction protocol.
Our case presentation confirms T. mycotoxinivorans as a potential emerging pathogen in patients with CF. However, our CF patient showed mild symptoms over a very long time period of 9 years. A short MALDI-TOF MS procedure allows reliable and rapid identification of T. mycotoxinivorans and therefore should facilitate further study on the clinical relevance and epidemiology of this unusual fungal organism.
食真菌毒素毛孢子菌是一种最近被描述的酵母样真菌，此前有报道称其与囊性纤维化（CF）患者的病原体有关。我们展示了一名CF患者9年的临床病程以及这种罕见真菌的现代分子和蛋白质组学鉴定技术的应用。
我们报告了一名32岁男性CF患者的病例，其痰培养在9年中持续被无性型酵母食真菌毒素毛孢子菌呈阳性。此外，还对食真菌毒素毛孢子菌进行了不同抗真菌药物的药敏试验。另外，使用简单的提取方案，应用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）对这种新型真菌病原体进行了快速鉴定方法。
我们的病例报告证实食真菌毒素毛孢子菌是CF患者中一种潜在的新兴病原体。然而，我们的CF患者在长达9年的时间里表现出轻微症状。简短的MALDI-TOF MS程序能够可靠且快速地鉴定食真菌毒素毛孢子菌，因此应有助于进一步研究这种不寻常真菌的临床相关性和流行病学。
