Paik Donggi, Monahan Amanda, Caffrey Daniel R, Elling Roland, Goldman William E, Silverman Neal
Program in Innate Immunity, Division of Infectious Diseases and Immunology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605; and.
Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599.
J Immunol. 2017 Jul 1;199(1):263-270. doi: 10.4049/jimmunol.1600409. Epub 2017 May 24.
Tracheal cytotoxin (TCT), a monomer of DAP-type peptidoglycan from , causes cytopathology in the respiratory epithelia of mammals and robustly triggers the Imd pathway. PGRP-LE, a cytosolic innate immune sensor in , directly recognizes TCT and triggers the Imd pathway, yet the mechanisms by which TCT accesses the cytosol are poorly understood. In this study, we report that CG8046, a SLC46 family transporter, is a novel transporter facilitating cytosolic recognition of TCT, and plays a crucial role in protecting flies against systemic infection. In addition, mammalian SLC46A2s promote TCT-triggered NOD1 activation in human epithelial cell lines, indicating that SLC46As is a conserved group of peptidoglycan transporter contributing to cytosolic immune recognition.
气管细胞毒素（TCT）是一种来自[具体来源未提及]的二氨基庚二酸型肽聚糖单体，可在哺乳动物呼吸道上皮细胞中引起细胞病变，并强烈激活免疫缺陷（Imd）途径。PGRP-LE是[具体物种未提及]中的一种胞质内天然免疫传感器，可直接识别TCT并激活Imd途径，但TCT进入细胞质的机制尚不清楚。在本研究中，我们报告称，CG8046，一种[具体物种未提及]的SLC46家族转运蛋白，是促进TCT胞质识别的新型转运蛋白，在保护果蝇免受全身性[具体感染类型未提及]感染方面发挥关键作用。此外，哺乳动物SLC46A2可促进人上皮细胞系中TCT触发的NOD1激活，表明SLC46A是一组保守的肽聚糖转运蛋白，有助于胞质免疫识别。
