[植物名称]花朵及分离出的黄酮类化合物的抗原虫、抗菌和抗腹泻特性 。 需注意，这里原文中[植物名称]部分缺失具体信息。
Antiprotozoal, Antibacterial and Antidiarrheal Properties from the Flowers of and Isolated Flavonoids.
作者信息
Calzada Fernando, Juárez Teresa, García-Hernández Normand, Valdes Miguel, Ávila Oscar, Mulia Lilian Yepez, Velázquez Claudia
机构信息
Unidad de Investigación Médica en Farmacología, UMAE Hospital de Especialidades-2° piso CORCE Centro Médico Nacional Siglo XXI, IMSS, Av. Cuauhtemoc, Col. Doctores, CP, Cd. México, México.
Unidad de Investigación en Epidemiología Clínica, IMSS, Hospital General Regional No 1 Carlos Mcgregor Sánchez Navarro, Gabriel Mancera. Colonia Del Valle Centro. Delegación, Benito Juárez, Cd. México CP & FES Zaragoza, Universidad Nacional Autónoma de México. Batalla 5 de mayo s/n. Colonia Ejército de Oriente. CP, Cd. Mexico, México.
出版信息
Pharmacogn Mag. 2017 Apr-Jun;13(50):240-244. doi: 10.4103/0973-1296.204564. Epub 2017 Apr 18.
BACKGROUND
Larreat. (Sterculiaceae) is a Mexican plant used in traditional medicine for the treatment of heart disease symptoms and infectious diarrhea.
OBJECTIVE
To evaluate antiprotozoal and antibacterial activities and antidiarrheal activity from the flowers of using the extract, fractions, and major isolated flavonoids.
MATERIALS AND METHODS
Bioassay-guided fractionation of the methanol extract of (MECP) led to the isolation of five flavonoids, tiliroside, astragalin, isoquercitrin, (+)-catechin, and (-)-epicatechin. Antimicrobial activities were tested on two protozoa ( and ) and nine bacterial enteropathogens (two strains, two strains, two strains, two sp. strains, and ) isolated from feces of children with acute diarrhea or dysentery and resistant to chloramphenicol. Also, antidiarrheal activity was tested on cholera toxin-induced diarrhea in male Balb-c mice.
RESULTS
Epicatechin was the most potent antiamoebic and antigiardial compound with IC values of 1.9 μg/mL for and 1.6 μg/mL for ; tiliroside showed moderate antiprotozoal activity against both protozoan. In contrast, in the antibacterial activity, tiliroside was the most potent compound on all microorganisms with minimum inhibitory concentration values less than 0.7 mg/mL. In the case of cholera toxin-induced diarrhea, epicatechin was the most potent flavonoid with IC of 14.7 mg/kg.
CONCLUSION
Epicatechin and tiliroside were the flavonoids responsible for antimicrobial andantidiarrheal activities of . Its antiprotozoal, antibacterial, and antidiarrheal properties are in good agreement with the traditional medicinal use of for the treatment of infectious diarrhea.
SUMMARY
Epicatechin was the most potent antiamoebic and antigiardial compound with IC values of 1.9 μg/mL for and 1.6 μg/mL for .Tiliroside showed antibacterial activity against all microorganisms tested with MIC values less than 0.7 mg/mL.Epicatechin was the most potent flavonoid on cholera toxin-induced diarrhea with IC of 14.7 mg/kg. MECP: Methanol extract of .
背景
Larreat.（梧桐科）是一种墨西哥植物，在传统医学中用于治疗心脏病症状和感染性腹泻。
目的
使用提取物、馏分和主要分离的黄酮类化合物评估其花的抗原虫、抗菌活性和抗腹泻活性。
材料与方法
对Larreat.甲醇提取物（MECP）进行生物测定导向的分级分离，得到5种黄酮类化合物，椴树苷、黄芪苷、异槲皮苷、（+）-儿茶素和（-）-表儿茶素。对从急性腹泻或痢疾患儿粪便中分离出且对氯霉素耐药的两种原生动物（溶组织内阿米巴和贾第虫）和9种细菌性肠道病原体（两种大肠杆菌菌株、两种志贺氏菌菌株、两种沙门氏菌菌株、两种弯曲杆菌属菌株和霍乱弧菌）进行抗菌活性测试。此外，对雄性Balb-c小鼠霍乱毒素诱导的腹泻进行抗腹泻活性测试。
结果
表儿茶素是最有效的抗阿米巴和抗贾第虫化合物，对溶组织内阿米巴的IC值为1.9μg/mL，对贾第虫的IC值为1.6μg/mL；椴树苷对两种原生动物均表现出中等抗原虫活性。相比之下，在抗菌活性方面，椴树苷是对所有微生物最有效的化合物，最低抑菌浓度值小于0.7mg/mL。在霍乱毒素诱导的腹泻中，表儿茶素是最有效的黄酮类化合物，IC值为14.7mg/kg。
结论
表儿茶素和椴树苷是Larreat.具有抗菌和抗腹泻活性的黄酮类化合物。其抗原虫、抗菌和抗腹泻特性与Larreat.用于治疗感染性腹泻的传统药用用途高度一致。
总结
表儿茶素是最有效的抗阿米巴和抗贾第虫化合物，对溶组织内阿米巴的IC值为1.9μg/mL，对贾第虫的IC值为1.6μg/mL。椴树苷对所有测试微生物均表现出抗菌活性，MIC值小于0.7mg/mL。表儿茶素是对霍乱毒素诱导的腹泻最有效的黄酮类化合物，IC值为14.7mg/kg。MECP：Larreat.的甲醇提取物
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