Tůmová Lenka, Dučaiová Zuzana, Cheel José, Vokřál Ivan, Sepúlveda Beatriz, Vokurková Doris
Department of Pharmacognosy, Faculty of Pharmacy, Charles University, Heyrovského, Hradec Králové, Czech Republic.
Laboratory of Algal Biotechnology-Centre ALGATECH, Institute of Microbiology of the Czech Academy of Sciences, Opatovický mlýn, Třeboň, Czech Republic.
Pharmacogn Mag. 2017 Apr-Jun;13(50):260-264. doi: 10.4103/0973-1296.204558. Epub 2017 Apr 18.
is traditionally used in the form of tea (infusion), in the Andean region of South America, to treat various chronic diseases. However, the health-promoting properties of this herbal tea have not yet been extensively explored.
The free radical scavenging activity of infusion (ACI) was evaluated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical and superoxide anion radical assays. The activation of immune cells by ACI, as determined by cell surface cluster of differentiation 69 expression, was measured by flow cytometry. The qualitative polyphenolic composition of ACI was investigated by HPLC/PDA/ESI-MS, (High-performance liquid chromatography coupled with photodiode array detection and electrospray ionization - mass spectrometry) and the total content of polyphenols was estimated by spectrophotometric methods.
Eight polyphenols including chlorogenic acid, 6,8-di--hexosyl apigenin, isoorientin, orientin, dicaffeoylquinic acid, biochanin A--glucoside, biochanin A--(malonyl)-glucoside, and licoisoflavone A were tentatively identified in ACI. The total contents of phenols, flavonoids, and tannins in lyophilized ACI were 5.40 mg/100 mg ACI, 1.79 mg/100 mg ACI, and 1.76 mg/100 mg ACI, respectively. ACI, within the range of 25-400 μg/mL, scavenged DPPH and O by 15-90% and 20-88%, respectively. The human natural killer (NK) cells were substantially activated by ACI, whereas T cells and granulocytes were slightly stimulated.
Overall, the results demonstrate the free radical scavenging and immune-stimulating properties of ACI, and support, at least in part, its potential utilization as a functional herbal tea. for preventing chronic diseases and as a nonspecific immune stimulator during human immunosenescence.
The total contents of phenols, flavonoids, and tannins in infusion (ACI) were 5.40 mg/100 mg ACI, 1.79 mg/100 mg ACI, and 1.76 mg/100 mg ACI, respectively.Eight polyphenols including chlorogenic acid, 6,8-di-C-hexosyl apigenin, isoorientin, orientin, dicaffeoylquinic acid, biochanin A--glucoside, biochanin A--(malonyl)-glucoside, and licoisoflavone A were tentatively identified in ACI by HPLC/PDA/ESI-MS.ACI, within the range of 25-400 μg/ml, scavenged 1,1-diphenyl-2-picrylhydrazyl (DPPH) and O. by 15-90% and 20-88%, respectively.The human natural killer (NK) cells were substantially activated by ACI, whereas T cells and granulocytes were slightly stimulated. ESI: electrospray ionization, HPLC: high performance liquid chromatography, PDA: photodiode array detector, MS: mass spectrometry, MS/MS: tandem mass spectrometry, MW: molecular weight, m/z: mass-to-charge ratio, FITC: fluorescent isothiocyanate, PE: phycoerythrin.
在南美洲安第斯地区，传统上以茶（浸剂）的形式使用来治疗各种慢性疾病。然而，这种草药茶的健康促进特性尚未得到广泛研究。
通过1,1-二苯基-2-苦基肼（DPPH）自由基和超氧阴离子自由基测定法评估浸剂（ACI）的自由基清除活性。通过流式细胞术测量由ACI激活的免疫细胞，这由细胞表面分化簇69表达来确定。通过高效液相色谱/光电二极管阵列检测/电喷雾电离-质谱联用（HPLC/PDA/ESI-MS）研究ACI的定性多酚成分，并通过分光光度法估算多酚的总含量。
在ACI中初步鉴定出8种多酚，包括绿原酸、6,8-二-C-己糖基芹菜素、异荭草苷、荭草苷、二咖啡酰奎宁酸、鹰嘴豆芽素A-β-葡萄糖苷、鹰嘴豆芽素A-β-（丙二酰）-葡萄糖苷和甘草异黄酮A。冻干ACI中酚类、黄酮类和单宁的总含量分别为5.40毫克/100毫克ACI、1.79毫克/100毫克ACI和1.76毫克/100毫克ACI。在25-400微克/毫升范围内，ACI分别清除15%-90%的DPPH和20%-88%的超氧阴离子。人自然杀伤（NK）细胞被ACI显著激活，而T细胞和粒细胞受到轻微刺激。
总体而言，结果证明了ACI的自由基清除和免疫刺激特性，并至少部分支持其作为功能性草药茶预防慢性疾病以及在人类免疫衰老期间作为非特异性免疫刺激剂的潜在用途。
浸剂（ACI）中酚类、黄酮类和单宁的总含量分别为5.40毫克/100毫克ACI、1.79毫克/100毫克ACI和1.76毫克/100毫克ACI。通过HPLC/PDA/ESI-MS在ACI中初步鉴定出8种多酚，包括绿原酸、6,8-二-C-己糖基芹菜素、异荭草苷、荭草苷、二咖啡酰奎宁酸、鹰嘴豆芽素A-β-葡萄糖苷、鹰嘴豆芽素A-β-（丙二酰）-葡萄糖苷和甘草异黄酮A。在25-400微克/毫升范围内，ACI分别清除1,1-二苯基-2-苦基肼（DPPH）和超氧阴离子15%-90%和20%-88%。人自然杀伤（NK）细胞被ACI显著激活，而T细胞和粒细胞受到轻微刺激。ESI：电喷雾电离，HPLC：高效液相色谱，PDA：光电二极管阵列检测器，MS：质谱，MS/MS：串联质谱，MW：分子量，m/z：质荷比，FITC：异硫氰酸荧光素，PE：藻红蛋白
