Faraone Immacolata, Rai Dilip K, Russo Daniela, Chiummiento Lucia, Fernandez Eloy, Choudhary Alka, Milella Luigi
Department of Science, University of Basilicata, V.le dell'Ateneo Lucano 10, 85100 Potenza, Italy.
Spinoff BioActiPlant s.r.l., University of Basilicata, V.le dell'Ateneo Lucano 10, 85100 Potenza, Italy.
Plants (Basel). 2019 Aug 3;8(8):265. doi: 10.3390/plants8080265.
Oxidative stress is involved in different diseases, such as diabetes and neurodegenerative diseases. The genus includes about 70 species of flowering plant species; most of them are commonly used as food and in particular as a tea infusion in the Andean region of South America in folk medicine to treat various chronic diseases. Wedd. aerial parts were firstly analyzed for their in vitro antioxidant activity using different complementary assays. In particular, radical scavenging activity was tested against biological neutral radical DPPH; ferric reducing power and lipid peroxidation inhibitory capacity (FRAP and -Carotene Bleaching tests) were also determined. The Relative Antioxidant Capacity Index (RACI) was used to compare data obtained by different assays. Then, the inhibitory ability of samples was investigated against -amylase and -glucosidase enzymes involved in diabetes and against acetylcholinesterase and butyrylcholinesterase enzymes considered as strategy for the treatment of Parkinson's or Alzheimer's diseases. Moreover, the phytochemical profile of the sample showing the highest RACI (1.35) and interesting enzymatic activities (IC of 163.54 ± 9.72 and 215.29 ± 17.10 μg/mL in -glucosidase and acetylcholinesterase inhibition, respectively) was subjected to characterization and quantification of its phenolic composition using LC-MS/MS analysis. In fact, the ethyl acetate fraction derived from ethanol extract by liquid/liquid extraction showed 29 compounds, most of them are cinnamic acid derivatives, flavonoid derivatives, and a terpene. To the best of our knowledge, this is the first report about the evaluation of significant biological activities and phytochemical profile of , an important source of health-promoting phytochemicals.
氧化应激与多种疾病有关，如糖尿病和神经退行性疾病。该属包括约70种开花植物物种；其中大多数通常用作食物，特别是在南美洲安第斯地区作为茶泡饮，在民间医学中用于治疗各种慢性疾病。首先使用不同的互补分析方法对Wedd.地上部分的体外抗氧化活性进行了分析。特别是，针对生物中性自由基DPPH测试了自由基清除活性；还测定了铁还原能力和脂质过氧化抑制能力（FRAP和β-胡萝卜素漂白试验）。使用相对抗氧化能力指数（RACI）来比较不同分析方法获得的数据。然后，研究了样品对糖尿病相关的α-淀粉酶和α-葡萄糖苷酶以及被认为是治疗帕金森病或阿尔茨海默病策略的乙酰胆碱酯酶和丁酰胆碱酯酶的抑制能力。此外，对显示出最高RACI（1.35）和有趣酶活性（α-葡萄糖苷酶和乙酰胆碱酯酶抑制的IC分别为163.54±9.72和215.29±17.10μg/mL）的样品的植物化学特征进行了表征，并使用LC-MS/MS分析对其酚类成分进行了定量。事实上，通过液/液萃取从乙醇提取物中得到的乙酸乙酯馏分显示出29种化合物，其中大多数是肉桂酸衍生物、黄酮类衍生物和一种萜烯。据我们所知，这是关于评估一种重要的促进健康植物化学物质来源的显著生物活性和植物化学特征的第一份报告。
