Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, China.
Food Chem. 2012 Dec 15;135(4):2802-7. doi: 10.1016/j.foodchem.2012.07.076. Epub 2012 Jul 25.
In this study, a high performance liquid chromatography-photo diode array detection-electrospray ionization tandem mass spectrometry (HPLC-DAD/ESI-MS) with pre-column DPPH assay is developed for screening the antioxidant components in the fruits of Livistona chinensis R. Br. Accordingly, six antioxidative flavonoids are identified as orientin, isoorientin, vitexin, isovitexin, isorhamnetin-3-O-glucoside and tricin in methanolic extract of L. chinensis fruits, based on their mass spectra and fragmentation patterns. To the best of our knowledge, orientin, isoorientin, isovitexin and isorhamnetin-3-O-glucoside were found firstly in this plant. The free radical scavenging activity of the six antioxidants found is further examined by off-line DPPH assay. The results indicated that the free radical scavenging activity of orientin and isoorientin are stronger than those of two antioxidative drugs, vitamin C and baicalin. In addition, an HPLC-DAD method is firstly established for simultaneous determination of the six antioxidants in L. chinensis fruits. Tricin was found to be the major component in L. chinensis fruits.
本研究建立了一种采用高效液相色谱-二极管阵列检测-电喷雾串联质谱（HPLC-DAD/ESI-MS）结合柱前 DPPH 法测定来筛选菠叶海枣果实中抗氧化成分的方法。因此，根据其质谱和裂解模式，从菠叶海枣果实的甲醇提取物中鉴定出 6 种具有抗氧化作用的类黄酮：山柰酚-3-O-葡萄糖苷、木犀草素、异荭草苷、异荭草素、异鼠李素-3-O-葡萄糖苷和麦角固醇。据我们所知，山柰酚、异荭草素、异荭草素和异鼠李素-3-O-葡萄糖苷首次在这种植物中被发现。进一步通过离线 DPPH 法测定了这 6 种抗氧化剂的自由基清除活性。结果表明，山柰酚和异荭草素的自由基清除活性强于两种抗氧化药物维生素 C 和黄芩苷。此外，首次建立了一种同时测定菠叶海枣果实中 6 种抗氧化剂的高效液相色谱-二极管阵列检测法。结果表明，麦角固醇是菠叶海枣果实中的主要成分。
