Cheel José, Onofre Gabriela, Vokurkova Doris, Tůmová Lenka, Neugebauerová Jarmila
Department of Pharmacognosy, Faculty of Pharmacy, Charles University, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.
Pharmacogn Mag. 2010 Jan;6(21):26-33. doi: 10.4103/0973-1296.59963. Epub 2010 Feb 13.
A licorice infusion (LI) and its major constituents were investigated for their capacity to stimulate the activation and the cell cycle progression of human lymphocytes, measured by the CD69 expression and DNA content, respectively. The chemical profile of LI was determined by high-performance liquid chromatography-diode array detection (HPLC-DAD).
Two major components of LI were identified as liquiritin (1) and glycyrrhizin (2); total flavones and flavonols were shown as its minor constituents. The LI (100-800 mug/ml) stimulated the expression of CD69 on lymphocytes in a concentration-independent manner. Values of the activation index (AI) of total lymphocytes treated with LI (100-800 mug/ml) did not differ significantly among them (P < 0.05), but were 50% lower than the AI value exhibited by cells treated with phytohemagglutinin (PHA). The LI showed a similar effect on T cells, but on a lower scale. Compounds 1 and 2 (12-100 mug/ml) did not stimulate the CD69 expression on lymphocytes. The LI, 1 and 2 showed no meaningful effect on cell cycle progression of lymphocytes. The experimental data indicates that LI stimulates the activation of lymphocytes as a result of a proliferation-independent process. This finding suggests that LI could be considered as a potential specific immune stimulator.
研究了甘草浸液（LI）及其主要成分刺激人淋巴细胞活化和细胞周期进程的能力，分别通过CD69表达和DNA含量进行测定。LI的化学图谱通过高效液相色谱-二极管阵列检测（HPLC-DAD）确定。
LI的两种主要成分被鉴定为甘草苷（1）和甘草酸（2）；总黄酮和黄酮醇为其次要成分。LI（100 - 800μg/ml）以浓度非依赖性方式刺激淋巴细胞上CD69的表达。用LI（100 - 800μg/ml）处理的总淋巴细胞的活化指数（AI）值之间无显著差异（P < 0.05），但比用植物血凝素（PHA）处理的细胞所显示的AI值低50%。LI对T细胞显示出类似作用，但程度较低。化合物1和2（12 - 100μg/ml）未刺激淋巴细胞上CD69的表达。LI、1和2对淋巴细胞的细胞周期进程无显著影响。实验数据表明，LI通过非增殖过程刺激淋巴细胞活化。这一发现表明LI可被视为一种潜在的特异性免疫刺激剂。
