Anti-Quorum Sensing Activity of on and .

BACKGROUND

Quorum sensing (QS) plays an important role in the production of virulence factors and pathogenicity in , and the interruption of QS will be a hopeful pathway to combat bacterial infection.

OBJECTIVE

In this study, we selected (Thunb.) Vahl from traditional Chinese herbal medicines for its anti-QS activity.

MATERIALS AND METHODS

Anti-QS of extracts (FSE) was monitored using the 12472 bioassay. Standard methods were used to investigate the effects of FSE on QS-controlled virulence factors production, swimming motility, and biofilm establishment in PAO1.

RESULTS

FSE could obviously inhibit the violacein production in 12472 and also could inhibit quorum sensing-regulated virulence factors production and biofilm formation in in a concentration-dependent manner. The elastase activity and pyocyanin production were inhibited at a maximum of 40.97 and 47.58% when was grown in the presence of 0.25 g/mL FSE, which can also inhibit swimming motility of . The biofilm formation ability was decreased about 72.45% when in PAO1 cultured with the 0.25 g/mL FSE. The results suggested that FSE may be used as an alternative drug to control and handle harmful infections caused by bacterial pathogens based on QS inhibition.

SUMMARY

water extract could obviously inhibit the purple pigment production in 12472 water extract could inhibit QS-regulated virulence factors production and biofilm formation in . QS: Quorum sensing, , , FSE: extracts, 12472 12472, AIs: autoinducers, AHLs: -acyl-homoserinelactones, LB: Luria-Bertani, MICs: Minimum inhibitory concentrations, CFU: Colony-Forming Units, ATCC: American Type Culture Collection, PBS: phosphate buffered saline.