Nguyen Khanh, Sparks Jean, Omoruyi Felix
Department of Life Sciences, Texas A&M University-Corpus Christi, Texas, USA.
Pharmacognosy Res. 2017 Apr-Jun;9(2):156-160. doi: 10.4103/0974-8490.204641.
roots have been traditionally used in folk medicine, but the scientific basis is unclear.
To investigate the cytotoxicity, antioxidant, and immunomodulatory effects of root extract on human promyelocytic leukemia (HL-60) cells and HO-induced oxidative damaged HL-60 cells.
HL-60 cells were incubated with different concentrations of root extract, and cells were harvested for viability assays on day 3 and 7. Cytokine levels (interferon-gamma [IFN-γ], interleukin-2 [IL-2], and interleukin-10 [IL-10]) and antioxidant indexes (malondialdehyde [MDA], reduced glutathione [GSH], superoxide dismutase [SOD], and catalase [CAT]) in HO-induced-stressed HL-60 were measured after 2 days.
The viability of HL-60 challenged with HO declined by 42% compared to unstressed cells. After 7 days of incubation with 200 or 400 μg/mL , the viability of HL-60 cells was two-fold higher than the control. Stressed HL-60 cells treated with 100, 200, and 400 μg/mL reduced the lipid peroxidation by 12%-13%. We noted an increase in GSH levels, SOD and CAT activities in stressed HL-60 supplemented with 400 μg/mL root extract. Treatment with 400 μg/mL significantly ( < 0.05) increased IFN-γ and IL-2 in HO-challenged cells.
Our data do not support the use of the extract as an antiproliferation and differentiation therapy for acute promyelocytic leukemia. The protective function of root extract against oxidative stress could occur through increasing GSH and higher expression of antioxidant enzymes.
Findings from this study may not support the use of root extract as an antiproliferation and differentiation therapy for acute promyelocytic leukemiaOur data suggest that root extract may be a potential antioxidant with protective effect against the oxidation of reduced glutathione (GSH)Treatment with root extract may be effective in preventing oxidative damage through increasing the activities of antioxidant enzymes (superoxide dismutase [SOD] and catalase [CAT]) in acute promyelocytic leukemia cells.
传统上根被用于民间医学，但科学依据尚不清楚。
研究根提取物对人早幼粒细胞白血病（HL-60）细胞以及过氧化氢（HO）诱导的氧化损伤HL-60细胞的细胞毒性、抗氧化和免疫调节作用。
将HL-60细胞与不同浓度的根提取物一起孵育，在第3天和第7天收获细胞进行活力测定。在HO诱导应激的HL-60细胞中培养2天后，测量细胞因子水平（γ干扰素[IFN-γ]、白细胞介素-2[IL-2]和白细胞介素-10[IL-10]）以及抗氧化指标（丙二醛[MDA]、还原型谷胱甘肽[GSH]、超氧化物歧化酶[SOD]和过氧化氢酶[CAT]）。
与未受应激的细胞相比，受HO攻击的HL-60细胞活力下降了42%。与200或400μg/mL孵育7天后，HL-60细胞的活力比对照高出两倍。用100、200和400μg/mL处理应激的HL-60细胞可使脂质过氧化降低12%-13%。我们注意到，在补充了400μg/mL根提取物的应激HL-60细胞中，GSH水平、SOD和CAT活性有所增加。用400μg/mL处理可显著（P<0.05）增加受HO攻击细胞中的IFN-γ和IL-2。
我们的数据不支持将该提取物用作急性早幼粒细胞白血病的抗增殖和分化疗法。根提取物对氧化应激的保护作用可能通过增加GSH以及抗氧化酶的更高表达来实现。
本研究结果可能不支持将根提取物用作急性早幼粒细胞白血病的抗增殖和分化疗法。我们的数据表明，根提取物可能是一种潜在的抗氧化剂，对还原型谷胱甘肽（GSH）的氧化具有保护作用。用根提取物处理可能通过增加急性早幼粒细胞白血病细胞中抗氧化酶（超氧化物歧化酶[SOD]和过氧化氢酶[CAT]）的活性来有效预防氧化损伤。
