Sudjarwo Sri Agus, Eraiko Koerniasari, Sudjarwo Giftania Wardani
Department of Pharmacology, Faculty of Veterinary Medicine, Airlangga University, Surabaya 60115, Indonesia.
Department of Conservative Dentistry, Faculty of Dentistry, Airlangga University, Surabaya 60115, Indonesia.
Pharmacognosy Res. 2017 Dec;9(Suppl 1):S5-S8. doi: 10.4103/pr.pr_66_17.
It has long been known that chickens, like mammals, are capable of producing antigen-specific immunoglobulin Y (IgY), which functions similar to IgG. The present study was performed to investigate the activity of IgY anti- on proliferation, interleukin (IL)-2, and interferon (IFN)-γ expression of rat peripheral blood mononuclear cells (PBMCs).
The activity of IgY anti- in different doses (25, 50, and 100 μg/ml) on rat PBMCs proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. The production of IL-2 and IFN-γ in the PBMC supernatant was determined using enzyme-linked immunosorbent assay. Investigation was performed on mRNA expression of IL-2 and IFN-γ by reverse transcription-polymerase chain reaction (RT-PCR).
IgY anti- significantly increased the proliferation of rat PBMC. Furthermore, IgY anti-M. dose dependently increased IL-2 and IFN-γ production in PBMC, suggesting that pharmacological activities of IgY anti- in PBMC may be mediated by regulating the production of cytokines. In the RT-PCR, expression of cytokines such as IL-2 and IFN-γ in PBMC cultures was increased by IgY anti-.
We concluded that increasing IL-2 and IFN-γ productions in PBMC was related to IgY anti-, stimulating the mRNA transcription (gene expression) of these cytokines which can induce proliferation of PBMC.
Lohman laying hens immunized intramuscularly with antigens of can produce specific IgY anti- complexIgY anti- significantly increased the proliferation of rat peripheral blood mononuclear cell (PBMC)IgY anti- dose dependently increased interleukin 2 (IL-2) and interferon (IFN)-γ production in PBMCIn the reverse transcription-polymerase chain reaction, expression of cytokines such as IL-2 and IFN-γ in PBMC cultures was increased by IgY anti-M. The increasing IL-2 and IFN-γ productions in PBMC were related to stimulation on mRNA transcription which can induce proliferation of PBMC. IgY anti-. tuberculosis: Immunoglobulin Y anti-; IL-2: Interleukin-2; IFN-γ: Interferon-γ; PBMCs: Peripheral blood mononuclear cells.
长期以来人们已知，鸡与哺乳动物一样，能够产生抗原特异性免疫球蛋白Y（IgY），其功能类似于IgG。本研究旨在探讨抗结核IgY对大鼠外周血单个核细胞（PBMC）增殖、白细胞介素（IL）-2及干扰素（IFN）-γ表达的影响。
采用3-(4,5-二甲基噻唑-2)-2,5-二苯基溴化四氮唑法测定不同剂量（25、50和100μg/ml）抗结核IgY对大鼠PBMC增殖的影响。采用酶联免疫吸附测定法检测PBMC上清液中IL-2和IFN-γ的产生情况。通过逆转录-聚合酶链反应（RT-PCR）研究IL-2和IFN-γ的mRNA表达。
抗结核IgY显著增加大鼠PBMC的增殖。此外，抗结核IgY剂量依赖性地增加PBMC中IL-2和IFN-γ的产生，提示抗结核IgY在PBMC中的药理活性可能通过调节细胞因子的产生来介导。在RT-PCR中，抗结核IgY增加了PBMC培养物中IL-2和IFN-γ等细胞因子的表达。
我们得出结论，PBMC中IL-2和IFN-γ产生的增加与抗结核IgY有关，抗结核IgY刺激这些可诱导PBMC增殖的细胞因子的mRNA转录（基因表达）。
用结核分枝杆菌抗原肌肉注射免疫罗曼蛋鸡可产生特异性抗结核IgY复合物。抗结核IgY显著增加大鼠外周血单个核细胞（PBMC）的增殖。抗结核IgY剂量依赖性地增加PBMC中白细胞介素2（IL-2）和干扰素（IFN）-γ的产生。在逆转录-聚合酶链反应中，抗结核IgY增加了PBMC培养物中IL-2和IFN-γ等细胞因子的表达。PBMC中IL-2和IFN-γ产生的增加与对可诱导PBMC增殖的mRNA转录的刺激有关。抗结核IgY。结核：免疫球蛋白Y抗结核；IL-2：白细胞介素-2；IFN-γ：干扰素-γ；PBMCs：外周血单个核细胞 。
