Department of Pathology, The University of the West Indies, Kingston, Jamaica.
University of Toronto, Toronto, Canada.
J Complement Integr Med. 2023 May 30;20(3):612-620. doi: 10.1515/jcim-2023-0048. eCollection 2023 Sep 1.
has beneficial properties. However, its cytotoxicity and antioxidative effects on human promyelocytic leukemia cells (HL60) deserve investigation. Therefore, the efficacy of its crude extracts in offsetting damage in HL60 cells subjected to oxidative stress was studied.
Crude extracts at different concentrations were incubated with HL60 cells. The beneficial properties of the plant extract against oxidative damage were evaluated post-induction of oxidative stress utilizing hydrogen peroxide.
Extracts at concentrations 600 and 800 μg/mL were most effective at increasing the viability of damaged cells compared to the control group after 48 h of incubation. Significant increases in lipid peroxidation were observed in exposed cells treated with 600 μg/mL extract after 72 h of incubation. Superoxide dismutase (SOD) and catalase activities significantly increased in exposed cells after 24 h of incubation at all extract concentrations. Exposed cells treated with 600 and 1,000 μg/dL of the extract showed significantly increased catalase activity after 48 h, and a similar profile was maintained after 72 h of exposure. SOD activity in exposed cells remained significantly increased at all treatment concentrations after 48 and 72 h of incubation. Treatment with 400, 600, and 800 μg/mL of the extract resulted in significantly increased reduced glutathione levels compared to the other groups after 24 and 72 h of incubation. However, after 48 h of incubation, significant increases were noted in glutathione levels in exposed cells incubated with either 400, 800, or 1,000 μg/mL extract.
The findings suggest that might effectively protect against oxidative damage in a time and extract concentration-dependent manner.
具有有益的特性。然而，其细胞毒性及其对人早幼粒细胞白血病细胞（HL60）的抗氧化作用仍有待研究。因此，研究了其粗提取物对氧化应激下 HL60 细胞损伤的抵消作用。
将不同浓度的粗提取物与 HL60 细胞孵育。在用过氧化氢诱导氧化应激后，评估植物提取物对氧化损伤的有益作用。
与对照组相比，孵育 48 小时后，浓度为 600 和 800μg/mL 的提取物最有效地增加了受损细胞的活力。孵育 72 小时后，暴露于细胞中的 600μg/mL 提取物的脂质过氧化显著增加。暴露于细胞中的超氧化物歧化酶（SOD）和过氧化氢酶活性在所有提取物浓度孵育 24 小时后均显著增加。暴露于细胞中的 600 和 1000μg/dL 提取物处理的细胞在孵育 48 小时后，过氧化氢酶活性显著增加，72 小时后仍保持类似的趋势。暴露于细胞中的 SOD 活性在孵育 48 和 72 小时后，在所有处理浓度下均保持显著增加。与其他组相比，孵育 24 和 72 小时后，用 400、600 和 800μg/mL 提取物处理的细胞中的还原型谷胱甘肽水平显著增加。然而，在孵育 48 小时后，暴露于细胞中用 400、800 或 1000μg/mL 提取物孵育的细胞中的谷胱甘肽水平显著增加。
研究结果表明，在时间和提取物浓度依赖的方式下，可能有效地保护细胞免受氧化损伤。
