使用L-半胱氨酸自组装单分子层修饰的微悬臂梁传感器对铜进行超灵敏检测。

Ultrasensitive Detection of Cu Using a Microcantilever Sensor Modified with L-Cysteine Self-Assembled Monolayer.

作者信息

Xu Xiaohe, Zhang Na, Brown Gilbert M, Thundat Thomas G, Ji Hai-Feng

机构信息

Department of Chemistry, Drexel University, Philadelphia, PA, 19104, USA.

Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.

出版信息

Appl Biochem Biotechnol. 2017 Oct;183(2):555-565. doi: 10.1007/s12010-017-2511-7. Epub 2017 May 24.

DOI:10.1007/s12010-017-2511-7

PMID:28540518

Abstract

A microcantilever was modified with a self-assembled monolayer (SAM) of L-cysteine for the sensitively and selectively response to Cu(II) ions in aqueous solution. The microcantilever undergoes bending due to sorption of Cu(II) ions. The interaction of Cu(II) ions with the L-cysteine on the cantilever is diffusion controlled and does not follow a simple Langmuir adsorption model. A concentration of 10 M Cu(II) was detected in a fluid cell using this technology. Other cations, such as Ni, Zn, Pb, Cd, Ca, K, and Na, did not respond with a significant deflection, indicating that this L-cysteine-modified cantilever responded selectively and sensitively to Cu(II).

摘要

用L-半胱氨酸自组装单分子层（SAM）修饰微悬臂梁，以实现对水溶液中Cu(II)离子的灵敏且选择性响应。由于Cu(II)离子的吸附，微悬臂梁会发生弯曲。Cu(II)离子与悬臂梁上L-半胱氨酸的相互作用受扩散控制，并不遵循简单的朗缪尔吸附模型。使用该技术在流体池中检测到了浓度为10 M的Cu(II)。其他阳离子，如Ni、Zn、Pb、Cd、Ca、K和Na，未引起明显的偏转响应，这表明这种L-半胱氨酸修饰的悬臂梁对Cu(II)具有选择性和灵敏的响应。