Serrano Antonio, Contreras Carmen, Ruiz-Filippi Gonzalo, Borja Rafael, Fermoso Fernando G
a Instituto de la Grasa (C.S.I.C.) , Sevilla , Spain.
b School of Biochemical Engineering , Pontificia Universidad Católica de Valparaíso , Valparaiso , Chile.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2017 Aug 24;52(10):986-991. doi: 10.1080/10934529.2017.1324711. Epub 2017 May 25.
The main objective of this study was to evaluate the suitability of Nannochloropsis gaditana to grow by sequential adaptation to TOPW (Table olive processing water) at increased substrate concentrations (10-80%). Sequential adaptation allows growing Nannochloropsis gaditana up to 80% TOPW, although the maximum microalgae biomass productions were achieved for percentages of 20-40%, i.e. 0.308 ± 0.005 g VSS (Volatile Suspended Solids)/L. In all growth experiments, proteins were the majority compound in the grown microalgae biomass (0.44 ± 0.05 g/g VSS), whereas phenols were retained up to a mean concentration of 12.1 ± 1.9 mg total phenols/g VSS. The highest microalgae biomass production rate at rate of 80% TOPW took place in the first two days when most nutrients were also removed. Average removal efficiencies at this percentage of TOPW were 69.1%, 50.9%, 54.3% and 71.8% for total organic carbon, total soluble nitrogen, phosphate and total phenols, respectively. Sequential adaptation can ensure the obtaining of a sustainable microalgae culture as a treatment method for TOPW.
本研究的主要目的是评估通过在增加底物浓度（10%-80%）的情况下对油橄榄加工废水（TOPW）进行连续驯化，来培养加的夫微拟球藻的适用性。连续驯化能够使加的夫微拟球藻在高达80%的TOPW中生长，不过在20%-40%的比例下可实现最大微藻生物量产量，即0.308±0.005克挥发性悬浮固体（VSS）/升。在所有生长实验中，蛋白质是所培养微藻生物量中的主要成分（0.44±0.05克/克VSS），而酚类物质的残留平均浓度为12.1±1.9毫克总酚/克VSS。在80%的TOPW比例下，微藻生物量的最高生产率出现在前两天，此时大多数营养物质也被去除。在该TOPW比例下，总有机碳、总可溶性氮、磷酸盐和总酚的平均去除效率分别为69.1%、50.9%、54.3%和71.8%。连续驯化可确保获得可持续的微藻培养物，作为处理TOPW的一种方法。
