Durkin J R, Ogrich L, Morales-Neira D, Dundumalla S, Abdelmalek M
Department of Dermatology, Drexel University College of Medicine, Philadelphia, PA, USA.
Skin Res Technol. 2018 Feb;24(1):54-58. doi: 10.1111/srt.12389. Epub 2017 May 24.
BACKGROUND/PURPOSE: Full-field optical coherence tomography (FFOCT) is an emerging imaging technique for rapid histological analysis. As FFOCT is introduced into the Mohs workflow, it is important to document the effect of tissue freezing on FFOCT images and any effect FFOCT has on frozen sections. Our study aimed to evaluate the changes on FFOCT images after tissue freezing as well as FFOCT imaging effects on frozen sectioning.
Six normal skin specimens were imaged using FFOCT and subsequently frozen using a cryostat. The specimens were then reimaged using FFOCT and compared for any differences. To evaluate the effect of FFOCT imaging on frozen sections, five normal skin specimens were bisected and one half was imaged using FFOCT. Both halves underwent frozen sectioning and analysis.
Significant changes in the fat, sebaceous glands, eccrine glands, and dermal collage and minimal changes in the epidermis were seen after freezing. No effect on frozen sectioning was seen after FFOCT imaging.
As FFOCT is studied for use in Mohs, clinicians should be aware that freezing prior to imaging introduces significant artifact in the FFOCT image. If possible, specimens should undergo imaging prior to being frozen.
背景/目的：全场光学相干断层扫描（FFOCT）是一种用于快速组织学分析的新兴成像技术。随着FFOCT被引入莫氏手术流程，记录组织冷冻对FFOCT图像的影响以及FFOCT对冷冻切片的任何影响非常重要。我们的研究旨在评估组织冷冻后FFOCT图像的变化以及FFOCT成像对冷冻切片的影响。
使用FFOCT对六个正常皮肤标本进行成像，随后使用低温恒温器进行冷冻。然后使用FFOCT对标本重新成像并比较任何差异。为了评估FFOCT成像对冷冻切片的影响，将五个正常皮肤标本一分为二，其中一半使用FFOCT进行成像。两半均进行冷冻切片和分析。
冷冻后可见脂肪、皮脂腺、汗腺和真皮胶原发生显著变化，而表皮变化最小。FFOCT成像后未观察到对冷冻切片的影响。
在研究将FFOCT用于莫氏手术时，临床医生应意识到成像前冷冻会在FFOCT图像中引入显著伪影。如果可能，标本应在冷冻前进行成像。
