Thouvenin Olivier, Boccara Claude, Fink Mathias, Sahel Jose, Pâques Michel, Grieve Kate
Institut Langevin, Paris, France.
Vision Institute, UPMC Université Paris 6, Paris, France.
Invest Ophthalmol Vis Sci. 2017 Sep 1;58(11):4605-4615. doi: 10.1167/iovs.17-22375.
To use cell motility as a contrast agent in retinal explants.
Macaque and mouse retinal explants were imaged with high resolution full field optical coherence tomography (FFOCT) and dynamic FFOCT, coupled with fluorescence imaging.
Static and dynamic FFOCT create complementary contrast from different structures within a cell. When imaging in vitro samples, static FFOCT detects steep refractive index gradients to reveal stationary structures including fibers, vessels, collagen, and cell contours, while dynamic FFOCT emphasizes metabolic activity of moving structures that are mainly intracellular, thus creating or enhancing contrast in cells that were previously hidden in noise. Dynamic FFOCT enables detection of most of the retinal cell types in the ganglion cell, inner and outer nuclear layers, where static FFOCT contrast is too low in relation to the noise background.
Composite static and dynamic FFOCT provides a new kind of FFOCT image containing valuable information for imaging of retinal explants. It provides label-free en face images of living retinas, with a subcellular resolution. Dynamic FFOCT adds information about cell activity, which is of interest in longitudinal explant studies.
将细胞运动性用作视网膜外植体的造影剂。
使用高分辨率全场光学相干断层扫描（FFOCT）和动态FFOCT对猕猴和小鼠视网膜外植体进行成像，并结合荧光成像。
静态和动态FFOCT从细胞内的不同结构产生互补的对比度。在对体外样本进行成像时，静态FFOCT检测陡峭的折射率梯度以揭示包括纤维、血管、胶原蛋白和细胞轮廓在内的静止结构，而动态FFOCT则强调主要位于细胞内的移动结构的代谢活性，从而在先前隐藏于噪声中的细胞中产生或增强对比度。动态FFOCT能够检测神经节细胞层、内核层和外核层中的大多数视网膜细胞类型，而在这些区域，相对于噪声背景，静态FFOCT的对比度过低。
静态和动态FFOCT相结合提供了一种新型的FFOCT图像，其中包含用于视网膜外植体成像的有价值信息。它提供了具有亚细胞分辨率的活视网膜的无标记正面图像。动态FFOCT增加了有关细胞活性的信息，这在纵向外植体研究中具有重要意义。
