Vakulenko S B, Entina E G, Fomina I P, Navashin S M
Antibiot Khimioter. 1988 Dec;33(12):913-7.
Aminoglycoside resistance genes were cloned from transconjugates of aminoglycoside resistant clinical strains of gramnegative bacteria. The resistance determinant cloned from the strains of E. coli transferred kanamycin, monomycin and neomycin resistance to laboratory strains. It was shown that the cloned resistance gene encoded the type I 3'-aminoglycoside phosphotransferase. The results of spot and blot hybridization of the gramnegative bacteria clinical strains with the Bam HI-Pst I fragment of the cloned resistance determinant were indicative of wide-spread distribution of the APH 3' (I) and closely related genes in clinical microbial populations.
从革兰氏阴性菌的氨基糖苷类耐药临床菌株的转接合子中克隆出氨基糖苷类耐药基因。从大肠杆菌菌株中克隆出的耐药决定簇将卡那霉素、单霉素和新霉素耐药性转移至实验室菌株。结果表明，克隆出的耐药基因编码I型3'-氨基糖苷磷酸转移酶。革兰氏阴性菌临床菌株与克隆出的耐药决定簇的Bam HI-Pst I片段的斑点杂交和印迹杂交结果表明，APH 3'(I)及密切相关基因在临床微生物群体中广泛分布。
