Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Guwahati, Assam 781032, India.
Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Guwahati, Assam 781032, India.
Biomed Pharmacother. 2017 Aug;92:207-214. doi: 10.1016/j.biopha.2017.05.069. Epub 2017 May 22.
Breast cancer is the prime cause for cancer mortality in women worldwide. The importance of diverse natural and dietary agents to reduce the risk of developing breast cancer is well established. Berberine, a natural isoquinoline alkaloid found in many medicinal plants is widely used in traditional Indian and Chinese medicine. Because of its capability to seize the cell cycle and induce apoptosis of numerous malignant cells, berberine has received considerable attention as a potential anticancer agent. In the present study, breast cancer was induced in Sprague Dawley (SD) rats by intragastric administration of 7, 12-dimethylbenz[a]anthracene (DMBA) at a dose of 80mg/kg of body weight. Treatment of berberine (50mg/kg BW) to breast tumor bearing rats was found to be effective against DMBA induced mammary carcinoma. The increased levels of lipid peroxide (malonaldehyde), pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α), enzymatic antioxidants (SOD and CAT), non-enzymatic antioxidants (GSH and vitamin C) and transcription factor NF-κB were decreased significantly by administration of berberine. Furthermore, RT-PCR and western blot analysis showed the down-regulation of NF-κB and PCNA in breast tumors. Histopathological studies validated that berberine is effective against DMBA induced ductal carcinoma & invasive carcinoma. Altogether, these findings demonstrate the preventive role of berberine against DMBA induced mammary carcinoma in SD rats.
乳腺癌是全球女性癌症死亡的主要原因。多种天然和饮食因子在降低乳腺癌发病风险方面的重要性已得到充分证实。小檗碱是一种存在于许多药用植物中的天然异喹啉生物碱，在印度传统医学和中医药中被广泛应用。由于其能够阻止细胞周期并诱导许多恶性细胞凋亡，小檗碱作为一种潜在的抗癌药物受到了广泛关注。在本研究中，通过灌胃给予 7,12-二甲基苯并[a]蒽（DMBA），以 80mg/kg 体重的剂量诱导 Sprague Dawley（SD）大鼠乳腺癌。发现小檗碱（50mg/kgBW）对荷瘤大鼠的治疗对 DMBA 诱导的乳腺癌有效。给予小檗碱可显著降低脂质过氧化物（丙二醛）、促炎细胞因子（IL-1β、IL-6 和 TNF-α）、酶抗氧化剂（SOD 和 CAT）、非酶抗氧化剂（GSH 和维生素 C）和转录因子 NF-κB 的水平。此外，RT-PCR 和 Western blot 分析显示 NF-κB 和 PCNA 在乳腺肿瘤中的下调。组织病理学研究证实小檗碱对 DMBA 诱导的导管癌和浸润性癌有效。总之，这些发现表明小檗碱对 SD 大鼠 DMBA 诱导的乳腺癌具有预防作用。
