Arens M, Swierkosz E, Dilworth V
Department of Pediatrics/Adolescent Medicine, St. Louis University School of Medicine, Missouri.
Diagn Microbiol Infect Dis. 1988 Nov;11(3):137-43. doi: 10.1016/0732-8893(88)90015-6.
We have analyzed frozen and fresh specimens for herpes simplex virus (HSV) to determine the effect of sonication and centrifugation on virus recovery. After sonication, titers of 24/27 specimens increased from 1.3-30.8-fold with a mean increase of 6.8-fold. One hundred seventy-four fresh specimens were inoculated before and after sonication into CV-1 tube cultures. There was not a statistically significant difference in time to positivity between the sonicated and unsonicated portions. Thus, although sonication of specimens can sharply increase the viral titer of positive specimens, sonication of fresh specimens does not significantly enhance the isolation of HSV. To determine the effect of centrifugation of clinical specimens on recovery of HSV, thirty-one culture positive frozen specimens were centrifuged, and the supernatants and resuspended sonicated pellets were titered. Twenty-five specimens had sufficient recoverable virus to titer; in 21/25 (87.5%) specimens the titers were reduced in the supernatants after centrifugation (mean reduction 38%; range of 4-92%). Two of 31 (7%) supernatants were negative in culture while the sonicated pellets were positive. Thus, centrifugation of specimens prior to cell inoculation may compromise recovery of HSV.
我们分析了冷冻和新鲜标本中的单纯疱疹病毒（HSV），以确定超声处理和离心对病毒回收率的影响。超声处理后，27份标本中有24份的滴度提高了1.3至30.8倍，平均提高了6.8倍。174份新鲜标本在超声处理前后接种到CV-1管培养物中。超声处理部分和未超声处理部分的阳性时间没有统计学上的显著差异。因此，尽管对标本进行超声处理可大幅提高阳性标本的病毒滴度，但对新鲜标本进行超声处理并不会显著提高HSV的分离率。为了确定对临床标本进行离心对HSV回收率的影响，对31份培养阳性的冷冻标本进行了离心，并对上清液和重新悬浮的超声处理沉淀物进行了滴定。25份标本有足够的可回收病毒进行滴定；在25份标本中的21份（87.5%）中，离心后上清液中的滴度降低（平均降低38%；范围为4-92%）。31份上清液中有2份（7%）在培养中呈阴性，而超声处理的沉淀物呈阳性。因此，在细胞接种前对标本进行离心可能会影响HSV的回收率。
