Sanders C, Nelson C, Hove M, Woods G L
Department of Pathology, University of Texas Medical Branch, Galveston 77555-0740, USA.
Diagn Microbiol Infect Dis. 1998 Oct;32(2):111-3. doi: 10.1016/s0732-8893(98)00075-3.
The reliability of a cytospin-enhanced direct immunofluorescence assay (DFA), using Syva Micro Trak monoclonal antibodies, for detection of herpes simplex virus (HSV) was evaluated by comparing results with those of conventional cell culture. Of 250 clinical specimens, 64 were positive for HSV by cell culture, and 22 were positive by cytospin-enhanced DFA, two of which were negative by culture. The sensitivity, specificity, and positive and negative predictive values for cytospin-enhanced DFA were 31.25, 98.9, 90.9, and 80.7%, respectively. Cytospin-enhanced DFA is not an acceptable alternative to cell culture for detection of HSV.
通过将结果与传统细胞培养的结果进行比较，评估了使用Syva Micro Trak单克隆抗体的细胞离心涂片增强直接免疫荧光测定法（DFA）检测单纯疱疹病毒（HSV）的可靠性。在250份临床标本中，64份通过细胞培养检测出HSV呈阳性，22份通过细胞离心涂片增强DFA检测呈阳性，其中两份通过培养检测为阴性。细胞离心涂片增强DFA的敏感性、特异性、阳性预测值和阴性预测值分别为31.25%、98.9%、90.9%和80.7%。对于HSV检测，细胞离心涂片增强DFA不是细胞培养可接受的替代方法。
