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1
Centrifugation-shell vial technique for rapid detection of herpes simplex virus cytopathic effect in Vero cells.用于在Vero细胞中快速检测单纯疱疹病毒细胞病变效应的离心管瓶技术
J Clin Microbiol. 1987 Feb;25(2):423-4. doi: 10.1128/jcm.25.2.423-424.1987.
2
Comparison of Chemicon SimulFluor direct fluorescent antibody staining with cell culture and shell vial direct immunoperoxidase staining for detection of herpes simplex virus and with cytospin direct immunofluorescence staining for detection of varicella-zoster virus.将Chemicon SimulFluor直接荧光抗体染色法与用于检测单纯疱疹病毒的细胞培养和空斑小瓶直接免疫过氧化物酶染色法以及用于检测水痘-带状疱疹病毒的细胞离心涂片直接免疫荧光染色法进行比较。
Clin Diagn Lab Immunol. 2001 Sep;8(5):909-12. doi: 10.1128/CDLI.8.5.909-912.2001.
3
Detection and serotyping of herpes simplex virus in MRC-5 cells by use of centrifugation and monoclonal antibodies 16 h postinoculation.接种后16小时，通过离心和单克隆抗体在MRC-5细胞中检测单纯疱疹病毒并进行血清分型。
J Clin Microbiol. 1985 Jan;21(1):29-32. doi: 10.1128/jcm.21.1.29-32.1985.
4
Comparison of enzyme immunoassay, shell vial culture, and conventional cell culture for the rapid detection of herpes simplex virus.酶免疫测定、空斑小室培养和传统细胞培养用于快速检测单纯疱疹病毒的比较
Diagn Microbiol Infect Dis. 1990 May-Jun;13(3):241-4. doi: 10.1016/0732-8893(90)90066-5.
5
Laboratory diagnosis of herpes simplex virus type 1 and type 2 infections.1型和2型单纯疱疹病毒感染的实验室诊断
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Evaluation of a rapid latex slide agglutination test for herpes simplex virus as a specimen screen and culture identification method.评价一种用于单纯疱疹病毒的快速乳胶玻片凝集试验作为标本筛查和培养鉴定方法的效果。
J Clin Microbiol. 1987 May;25(5):936-7. doi: 10.1128/jcm.25.5.936-937.1987.
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Rapid detection of herpes simplex virus in clinical specimens by centrifugation and immunoperoxidase staining.通过离心和免疫过氧化物酶染色快速检测临床标本中的单纯疱疹病毒。
J Clin Microbiol. 1986 Apr;23(4):683-6. doi: 10.1128/jcm.23.4.683-686.1986.
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Continuous high-speed rolling versus centrifugation for detection of herpes simplex virus.连续高速滚动法与离心法检测单纯疱疹病毒的比较
J Clin Microbiol. 1989 Dec;27(12):2884-6. doi: 10.1128/jcm.27.12.2884-2886.1989.
10
Evaluation of an enzyme immunoassay for detection of herpes simplex virus antigen in genital lesions.用于检测生殖器病变中单纯疱疹病毒抗原的酶免疫测定法的评估。
Eur J Clin Microbiol. 1987 Aug;6(4):410-3. doi: 10.1007/BF02013096.

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Herpes simplex virus type 1 is the leading cause of genital herpes in New Brunswick.单纯疱疹病毒 1 型是新不伦瑞克省生殖器疱疹的主要病因。
Can J Infect Dis Med Microbiol. 2012 Spring;23(1):15-8. doi: 10.1155/2012/856759.
2
Physical and chemical methods for enhancing rapid detection of viruses and other agents.用于加强病毒及其他病原体快速检测的物理和化学方法。
Clin Microbiol Rev. 1993 Apr;6(2):150-75. doi: 10.1128/CMR.6.2.150.
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Viral infections in severely immunocompromised cancer patients.严重免疫功能低下癌症患者的病毒感染
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Effect of high-speed rolling on herpes simplex virus detection and replication.高速滚动对单纯疱疹病毒检测与复制的影响。
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5
Modified spin-amplified adsorption procedure with conventional tissue culture tubes for rapid detection and increased recovery of herpes simplex virus from clinical specimens.采用常规组织培养管的改良自旋放大吸附程序，用于从临床标本中快速检测和提高单纯疱疹病毒的回收率。
J Clin Microbiol. 1988 Oct;26(10):2195-9. doi: 10.1128/jcm.26.10.2195-2199.1988.
6
Conventional tube cell culture compared with centrifugal inoculation of MRC-5 cells and staining with monoclonal antibodies for detection of herpes simplex virus in clinical specimens.传统的管细胞培养与MRC-5细胞的离心接种以及用单克隆抗体染色用于检测临床标本中的单纯疱疹病毒的比较。
J Clin Microbiol. 1988 Mar;26(3):570-2. doi: 10.1128/jcm.26.3.570-572.1988.
7
Role of the virology laboratory in diagnosis and management of patients with central nervous system disease.病毒学实验室在中枢神经系统疾病患者诊断与管理中的作用。
Clin Microbiol Rev. 1989 Jan;2(1):1-14. doi: 10.1128/CMR.2.1.1.
8
Continuous high-speed rolling versus centrifugation for detection of herpes simplex virus.连续高速滚动法与离心法检测单纯疱疹病毒的比较
J Clin Microbiol. 1989 Dec;27(12):2884-6. doi: 10.1128/jcm.27.12.2884-2886.1989.
9
Detection of herpes simplex virus by 8 h in shell vial cultures with primary rabbit kidney cells.在原代兔肾细胞空斑试验培养物中8小时内检测单纯疱疹病毒。
J Clin Microbiol. 1989 Jan;27(1):199-200. doi: 10.1128/jcm.27.1.199-200.1989.
10
Comparison of primary rabbit kidney and MRC-5 cells and two stain procedures for herpes simplex virus detection by a shell vial centrifugation method.采用空斑小瓶离心法检测单纯疱疹病毒时，原代兔肾细胞和MRC-5细胞的比较及两种染色程序
J Clin Microbiol. 1988 Feb;26(2):222-4. doi: 10.1128/jcm.26.2.222-224.1988.

本文引用的文献

1
Herpes simplex infection at term. What to do with mother, newborn, and nursery personnel.足月时的单纯疱疹感染。如何处理母亲、新生儿及产房工作人员的问题。
JAMA. 1980 Jan 11;243(2):157-60. doi: 10.1001/jama.243.2.157.
2
Rapid detection of herpes simplex virus in clinical specimens with human embryonic lung fibroblast and primary rabbit kidney cell cultures.用人胚肺成纤维细胞和原代兔肾细胞培养物快速检测临床标本中的单纯疱疹病毒
J Clin Microbiol. 1984 Apr;19(4):563-5. doi: 10.1128/jcm.19.4.563-565.1984.
3
Comparison of human fibroblast cells and primary rabbit kidney cells for isolation of herpes simplex virus.用于单纯疱疹病毒分离的人成纤维细胞与原代兔肾细胞的比较。
J Clin Microbiol. 1984 Apr;19(4):548-9. doi: 10.1128/jcm.19.4.548-549.1984.
4
Comparison of direct immunofluorescence and direct immunoperoxidase procedures for detection of herpes simplex virus antigen in lesion specimens.用于检测病变标本中单纯疱疹病毒抗原的直接免疫荧光法与直接免疫过氧化物酶法的比较。
J Clin Microbiol. 1983 Aug;18(2):445-8. doi: 10.1128/jcm.18.2.445-448.1983.
5
Direct immunofluorescence staining for detection of herpes simplex and varicella-zoster virus antigens in vesicular lesions and certain tissue specimens.用于检测水疱性病变和某些组织标本中单纯疱疹病毒和水痘-带状疱疹病毒抗原的直接免疫荧光染色。
J Clin Microbiol. 1980 Nov;12(5):651-5. doi: 10.1128/jcm.12.5.651-655.1980.
6
Risk of recurrence after first episodes of genital herpes. Relation to HSV type and antibody response.生殖器疱疹首次发作后的复发风险。与单纯疱疹病毒类型及抗体反应的关系。
N Engl J Med. 1981 Aug 6;305(6):315-9. doi: 10.1056/NEJM198108063050604.
7
Drug therapy: antiviral agents (second of two parts).药物治疗：抗病毒药物（两部分中的第二部分）
N Engl J Med. 1980 Apr 24;302(17):949-53. doi: 10.1056/NEJM198004243021705.
8
Comparison of standard tissue culture, tissue culture plus staining, and direct staining for detection of genital herpes simplex virus infection.标准组织培养、组织培养加染色及直接染色检测生殖器单纯疱疹病毒感染的比较
J Clin Microbiol. 1984 May;19(5):631-3. doi: 10.1128/jcm.19.5.631-633.1984.
9
Rapid detection and identification of herpes simplex virus in cell culture by a direct immunoperoxidase staining procedure.通过直接免疫过氧化物酶染色法在细胞培养中快速检测和鉴定单纯疱疹病毒。
J Clin Microbiol. 1983 Sep;18(3):550-3. doi: 10.1128/jcm.18.3.550-553.1983.
10
Evaluation of a commercial enzyme-linked immunosorbent assay for detection of herpes simplex virus antigen.用于检测单纯疱疹病毒抗原的一种商用酶联免疫吸附测定法的评估
J Clin Microbiol. 1984 Sep;20(3):490-3. doi: 10.1128/jcm.20.3.490-493.1984.

用于在Vero细胞中快速检测单纯疱疹病毒细胞病变效应的离心管瓶技术

Centrifugation-shell vial technique for rapid detection of herpes simplex virus cytopathic effect in Vero cells.

作者信息

Pruneda R C, Almanza I

出版信息

J Clin Microbiol. 1987 Feb;25(2):423-4. doi: 10.1128/jcm.25.2.423-424.1987.

DOI:10.1128/jcm.25.2.423-424.1987
PMID:3029174
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC265913/
Abstract

A centrifugation-shell vial technique for herpes simplex virus detected the virus in 50 (24.6%) of 203 specimens by cytopathic effect at 48 h, compared with detection of the virus in 47 (23.2%) specimens by immunofluorescence at 24 h. The rapid detection of cytopathic effect may be useful to laboratories that are not interested in typing all herpes simplex virus specimens but that wish to reduce the cost of herpesvirus cultures.

摘要

一种用于检测单纯疱疹病毒的离心管瓶技术，在48小时时通过细胞病变效应在203份标本中的50份（24.6%）检测到了该病毒，相比之下，在24小时时通过免疫荧光在47份（23.2%）标本中检测到了该病毒。细胞病变效应的快速检测对于那些对所有单纯疱疹病毒标本进行分型不感兴趣但希望降低疱疹病毒培养成本的实验室可能有用。