Woods G L, Mills R D
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68105.
J Clin Microbiol. 1988 Jun;26(6):1233-5. doi: 10.1128/jcm.26.6.1233-1235.1988.
During a 4-month period, two methods for rapid detection of herpes simplex virus (HSV) were examined: (i) pretreatment of A549 cells with dexamethasone for conventional tissue culture (277 specimens) and (ii) 24-well plate centrifugation using A549 cells with and without dexamethasone pretreatment and staining with serotype-specific monoclonal antibodies (Syva Co., Palo Alto, Calif.) after incubation for 16 to 18 h (153 specimens). By conventional tube cell culture, both with and without dexamethasone, HSV was identified in 88 of 277 (32%) specimens. Significantly more specimens were positive for HSV at 24 h (46 versus 27 specimens) and at 48 h (a total of 72 versus 59 specimens) (P less than 0.0001) in dexamethasone-treated A549 cells. Of the 153 specimens tested by conventional culture and 24-well plate centrifugation, HSV was detected in 44 (29%) by conventional culture, and by 24-well plate centrifugation with and without dexamethasone, HSV was detected in 32 (21%) and 30 (20%) specimens, respectively. The sensitivity, specificity, and positive and negative predictive values of 24-well plate centrifugation with A549 cells for detection of HSV were 73 (71% without dexamethasone), 100, 100, and 90%, respectively. In conventional tube cell culture, pretreatment of A549 cells with dexamethasone results in more rapid detection of HSV. Centrifugal inoculation of dexamethasone-treated and untreated A549 cells in 24-well plates and staining with monoclonal antibodies after incubation for 16 to 18 h is an insensitive means to detect HSV in clinical specimens and should not replace conventional tube cell culture.
在4个月的时间里，对两种快速检测单纯疱疹病毒（HSV）的方法进行了检测：（i）用地塞米松预处理A549细胞用于传统组织培养（277份标本），以及（ii）使用经地塞米松预处理和未预处理的A549细胞进行24孔板离心，并在孵育16至18小时后用血清型特异性单克隆抗体（Syva公司，加利福尼亚州帕洛阿尔托）染色（153份标本）。通过传统的试管细胞培养，无论有无地塞米松，在277份标本中的88份（32%）中鉴定出HSV。在地塞米松处理的A549细胞中，在24小时时HSV阳性标本显著增多（46份对27份标本），在48小时时也显著增多（总共72份对59份标本）（P小于0.0001）。在通过传统培养和24孔板离心检测的153份标本中，通过传统培养在44份（29%）中检测到HSV，通过有地塞米松和无地塞米松的24孔板离心，分别在32份（21%）和30份（20%）标本中检测到HSV。用A549细胞进行24孔板离心检测HSV的敏感性、特异性、阳性和阴性预测值分别为73（无地塞米松时为71%）、100、100和90%。在传统的试管细胞培养中，用地塞米松预处理A549细胞可更快速地检测HSV。将经地塞米松处理和未处理的A549细胞接种到24孔板中进行离心，并在孵育16至18小时后用单克隆抗体染色，是一种检测临床标本中HSV的不敏感方法，不应取代传统的试管细胞培养。
